Enhancement of Nucleoside Production in Hirsutella sinensis Based on Biosynthetic Pathway Analysis

To enhance nucleoside production in Hirsutella sinensis, the biosynthetic pathways of purine and pyrimidine nucleosides were constructed and verified. The differential expression analysis showed that purine nucleoside phosphorylase, inosine monophosphate dehydrogenase, and guanosine monophosphate sy...

Full description

Saved in:
Bibliographic Details
Published in:BioMed research international 2017-01, Vol.2017 (2017), p.1-11
Main Authors: Teng, Yi, Wu, Ling-Fang, Zheng, Yu-Guo, Xu, Feng, Wu, Hui, Xue, Ya-Ping, Chen, Mao-Sheng, Baker, Peter James, Lin, Shan, Zhang, Bo, Liu, Zhi-Qiang, Yuan, Shui-Jin
Format: Article
Language:English
Subjects:
Adenosine
Ascomycota
Biology
Biosynthesis
Biotechnology
Carotenoids
Chromatography
Dehydrogenase
Dihydroorotate dehydrogenase
E coli
Enzymes
Fermentation
Fungi
Gene expression
Genes
Genomes
Genomics
Guanosine
Hirsutella sinensis
Inosine monophosphate
Metabolism
Nucleosides
Ophiocordyceps sinensis
Phosphorylase
Physiological aspects
Pyrophosphatase
Ribose
Studies
Uracil
Uridine
Uridine nucleosidase
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To enhance nucleoside production in Hirsutella sinensis, the biosynthetic pathways of purine and pyrimidine nucleosides were constructed and verified. The differential expression analysis showed that purine nucleoside phosphorylase, inosine monophosphate dehydrogenase, and guanosine monophosphate synthase genes involved in purine nucleotide biosynthesis were significantly upregulated 16.56-fold, 8-fold, and 5.43-fold, respectively. Moreover, dihydroorotate dehydrogenase, uridine nucleosidase, uridine/cytidine monophosphate kinase, and inosine triphosphate pyrophosphatase genes participating in pyrimidine nucleoside biosynthesis were upregulated 4.53-fold, 10.63-fold, 4.26-fold, and 5.98-fold, respectively. To enhance the nucleoside production, precursors for synthesis of nucleosides were added based on the analysis of biosynthetic pathways. Uridine and cytidine contents, respectively, reached 5.04 mg/g and 3.54 mg/g when adding 2 mg/mL of ribose, resulting in an increase of 28.6% and 296% compared with the control, respectively. Meanwhile, uridine and cytidine contents, respectively, reached 10.83 mg/g 2.12 mg/g when adding 0.3 mg/mL of uracil, leading to an increase of 176.3% and 137.1%, respectively. This report indicated that fermentation regulation was an effective way to enhance the nucleoside production in H. sinensis based on biosynthetic pathway analysis.
ISSN:2314-6133
2314-6141
DOI:10.1155/2017/2520347