Acquisition of the Phosphate Transporter NptA Enhances Staphylococcus aureus Pathogenesis by Improving Phosphate Uptake in Divergent Environments

• Published in: Infection and immunity 2018-01, Vol.86 (1)
• Main Authors: Kelliher, Jessica L, Radin, Jana N, Grim, Kyle P, Párraga Solórzano, Paola K, Degnan, Patrick H, Kehl-Fie, Thomas E
• Format: Article
• Language: English
• Subjects: Molecular Pathogenesis
• ISSN: 0019-9567; 1098-5522
• DOI: 10.1128/IAI.00631-17

During infection, pathogens must obtain all inorganic nutrients, such as phosphate, from the host. Despite the essentiality of phosphate for all forms of life, how obtains this nutrient during infection is unknown. Differing from , the paradigm for bacterial phosphate acquisition, which has two inorganic phosphate (P ) importers, genomic analysis suggested that possesses three distinct P transporters: PstSCAB, PitA, and NptA. While and are expressed in phosphate-replete media, expression of all three transporters is induced by phosphate limitation. The loss of a single transporter did not affect However, disruption of any two systems significantly reduced P accumulation and growth in divergent environments. These findings indicate that PstSCAB, PitA, and NptA have overlapping but nonredundant functions, thus expanding the environments in which can successfully obtain P Consistent with this idea, in a systemic mouse model of disease, loss of any one transporter did not decrease staphylococcal virulence. However, loss of NptA in conjunction with either PstSCAB or PitA significantly reduced the ability of to cause infection. These observations suggest that P acquisition via NptA is particularly important for the pathogenesis of While our analysis suggests that NptA homologs are widely distributed among bacteria, closely related less pathogenic staphylococcal species do not possess this importer. Altogether, these observations indicate that P uptake by differs from established models and that acquisition of a third transporter enhances the ability of the bacterium to cause infection.