Characterization of the Transcriptome and Gene Expression of Tetraploid Black Locust Cuttings in Response to Etiolation

Etiolation (a process of growing plants in partial or complete absence of light) promotes adventitious root formation in tetraploid black locust ( L.) cuttings. We investigated the mechanism underlying how etiolation treatment promotes adventitious root formation in tetraploid black locust and asses...

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Published in:Genes 2017-11, Vol.8 (12), p.345
Main Authors: Lu, Nan, Dai, Li, Luo, Zijing, Wang, Shaoming, Wen, Yanzhong, Duan, Hongjing, Hou, Rongxuan, Sun, Yuhan, Li, Yun
Format: Article
Language:English
Subjects:
Etiolation
Gene expression
Indoleacetic acid
Metabolism
Peroxidase
Photosynthesis
Polyphenol oxidase
Robinia pseudoacacia
Sucrose
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Summary:Etiolation (a process of growing plants in partial or complete absence of light) promotes adventitious root formation in tetraploid black locust ( L.) cuttings. We investigated the mechanism underlying how etiolation treatment promotes adventitious root formation in tetraploid black locust and assessed global transcriptional changes after etiolation treatment. Solexa paired-end sequencing of complementary DNAs (cDNAs) from control (non-etiolated, NE) and etiolated (E) samples resulted in 107,564 unigenes. In total, 52,590 transcripts were annotated and 474 transcripts (211 upregulated and 263 downregulated) potentially involved in etiolation were differentially regulated. These genes were associated with hormone metabolism and response, photosynthesis, signaling pathways, and starch and sucrose metabolism. In addition, we also found significant differences of phytohormone contents, activity of following enzymes i.e., peroxidase, polyphenol oxidase and indole acetic acid oxidase between NE and E tissues during some cottage periods. The genes responsive to etiolation stimulus identified in this study will provide the base for further understanding how etiolation triggers adventitious roots formation in tetraploid black locus.
ISSN:2073-4425
2073-4425
DOI:10.3390/genes8120345