Immortalization of Porcine 11β-Hydroxysteroid Dehydrogenase Type 1-Transgenic Liver Cells Using SV40 Large T Antigen

• Published in: International journal of molecular sciences 2017-12, Vol.18 (12), p.2625
• Main Authors: Kang, Hee Young, Choi, Young-Kwon, Jeong, Yeon Ik, Choi, Kyung-Chul, Hyun, Sang-Hwan, Hwang, Woo-Suk, Jeung, Eui-Bae
• Format: Article
• Language: English
• Subjects: 11-beta-Hydroxysteroid Dehydrogenase Type 1 - genetics; 11-beta-Hydroxysteroid Dehydrogenase Type 1 - metabolism; 11β-Hydroxysteroid dehydrogenase; Adipose tissue; Adipose Tissue - cytology; Animals; Antigens; Antigens, Viral, Tumor - genetics; Antigens, Viral, Tumor - metabolism; Blood glucose; Blood levels; Cell proliferation; Cell Proliferation - physiology; Cells, Cultured; Cortisone; Dehydrogenases; Fibroblasts; Hepatocytes; Hepatocytes - cytology; Hepatocytes - metabolism; Homeostasis; Hormones; Hydrocortisone; Hydroxysteroids; Hypoglycemia; Immortalization; Insulin; Life span; Liver; Liver - cytology; Metabolic syndrome; Metabolism; Rodents; Swine
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms18122625

Cortisol is a steroid hormone essential to the maintenance of homeostasis that is released in response to stress and low blood glucose concentration. Cortisol is converted from cortisone by 11βhydroxysteroid dehydrogenase type 1 (HSD11B1). It has been reported that too much cortisol or overexpression of HSD11B1 induces obesity and the insulin resistance that accompanies metabolic syndrome in rodent adipose tissue. In our previous study, -transgenic (TG) fibroblasts were established, and a porcine model was generated by SCNT using those fibroblasts. Hepatocytes overexpressing HSD11B1 were obtained from livers of this porcine model and cultured in vitro. However, the primary hepatocytes were found to have a short life span or low proliferation rate. To overcome these problems, the SV40 large T antigen was transduced into primary -TG hepatocytes, and those cells were immortalized. Immortalized -TG hepatocytes showed restored morphology, more rapid proliferation rate, and more expression of HSD11B1 than primary hepatocytes. As well, these cells kept the hepatic characteristics such as gluconeogenic response to cortisone and increased expression of hepatic makers. The immortalized -TG hepatocytes may be useful for studying traits and potential therapeutic drugs for treatment of metabolic disorders induced by overexpression of HSD11B1.