Cardiopulmonary Bypass in a Mouse Model: A Novel Approach

As prolonged cardiopulmonary bypass becomes more essential during cardiac interventions, an increasing clinical demand arises for procedure optimization and for minimizing organ damage resulting from prolonged extracorporal circulation. The goal of this paper was to demonstrate a fully functional an...

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Bibliographic Details
Published in:Journal of visualized experiments 2017-09 (127)
Main Authors: Madrahimov, Nodir, Natanov, Ruslan, Boyle, Erin C, Goecke, Tobias, Knöfel, Ann-Kathrin, Irkha, Valentyna, Solovieva, Anna, Höffler, Klaus, Maus, Ulrich, Kühn, Christian, Ismail, Issam, Warnecke, Gregor, Shrestha, Malakh-Lal, Cebotari, Serghei, Haverich, Axel
Format: Article
Language:English
Subjects:
Animals
Cardiopulmonary Bypass - methods
Disease Models, Animal
Extracorporeal Circulation - methods
Medicine
Mice
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Summary:As prolonged cardiopulmonary bypass becomes more essential during cardiac interventions, an increasing clinical demand arises for procedure optimization and for minimizing organ damage resulting from prolonged extracorporal circulation. The goal of this paper was to demonstrate a fully functional and clinically relevant model of cardiopulmonary bypass in a mouse. We report on the device design, perfusion circuit optimization, and microsurgical techniques. This model is an acute model, which is not compatible with survival due to the need for multiple blood drawings. Because of the range of tools available for mice (e.g., markers, knockouts, etc.), this model will facilitate investigation into the molecular mechanisms of organ damage and the effect of cardiopulmonary bypass in relation to other comorbidities.
ISSN:1940-087X
1940-087X
DOI:10.3791/56017