Dynamics of translation can determine the spatial organization of membrane-bound proteins and their mRNA

Unlike most macromolecules that are homogeneously distributed in the bacterial cell, mRNAs that encode inner-membrane proteins can be concentrated near the inner membrane. Cotranslational insertion of the nascent peptide into the membrane brings the translating ribosome and the mRNA close to the mem...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2017-12, Vol.114 (51), p.13424-13429
Main Authors: Korkmazhan, Elgin, Teimouri, Hamid, Peterman, Neil, Levine, Erel
Format: Article
Language:English
Subjects:
Bacteria
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Biological Sciences
Cell Membrane - metabolism
Macromolecules
Membrane proteins
Membrane Proteins - genetics
Membrane Proteins - metabolism
Models, Biological
Molecules
mRNA
Peptides
Physical Sciences
Post-transcription
Protein Biosynthesis
Protein Transport
Proteins
Ribonucleic acid
RNA
RNA, Messenger - genetics
RNA, Messenger - metabolism
Size distribution
Spatial distribution
Stochastic models
Stochasticity
Translation
Translation initiation
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Summary:Unlike most macromolecules that are homogeneously distributed in the bacterial cell, mRNAs that encode inner-membrane proteins can be concentrated near the inner membrane. Cotranslational insertion of the nascent peptide into the membrane brings the translating ribosome and the mRNA close to the membrane. This suggests that kinetic properties of translation can determine the spatial organization of these mRNAs and proteins, which can be modulated through posttranscriptional regulation. Here we use a simple stochastic model of translation to characterize the effect of mRNA properties on the dynamics and statistics of its spatial distribution. We show that a combination of the rate of translation initiation, the availability of secretory apparatuses, and the composition of the coding region determines the abundance of mRNAs near the membrane, as well as their residence time. We propose that the spatiotemporal dynamics of mRNAs can give rise to protein clusters on the membrane and determine their size distribution.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.1700941114