The construction and application of a cell line resistant to novel subgroup avian leukosis virus (ALV-K) infection

A novel avian leukosis viruses (ALV) subgroup named ALV-K was recently isolated from Chinese indigenous chickens which is different from the subgroups (A to E and J) that have previously been reported to infect chickens. More and more ALV-K strains have recently been isolated from local breeds of Ch...

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Bibliographic Details
Published in:Archives of virology 2018-01, Vol.163 (1), p.89-98
Main Authors: Mingzhang, Rao, Zijun, Zhao, Lixia, Yuan, Jian, Chen, Min, Feng, Jie, Zhang, Ming, Liao, Weisheng, Cao
Format: Article
Language:English
Subjects:
Animals
Avian leukosis
Avian Leukosis - virology
Avian leukosis virus
Avian Leukosis Virus - physiology
Biomedical and Life Sciences
Biomedicine
Cell Line
cell lines
Chickens
diagnostic techniques
Env gene
Fibroblasts - metabolism
Fibroblasts - virology
genes
Genetic Engineering
Humans
Infections
Infectious Diseases
Leukosis
Medical Microbiology
Molecular modelling
Original
Original Article
Poultry Diseases - virology
Proteins
Viral Envelope Proteins
Viral infections
Virology
viruses
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Summary:A novel avian leukosis viruses (ALV) subgroup named ALV-K was recently isolated from Chinese indigenous chickens which is different from the subgroups (A to E and J) that have previously been reported to infect chickens. More and more ALV-K strains have recently been isolated from local breeds of Chinese chickens. However, there are no more effective diagnostic methods for ALV-K other than virus isolation followed by envelope gene sequencing and comparison. Viral infection can be blocked through expression of the viral receptor-binding protein. In this study, we have engineered a cell line, DF-1/K, that expresses ALV-K env protein and thereby confers resistance to ALV-K infection. DF-1/K can be used in combination with the ALV-K susceptible cell line DF-1 as a specific diagnostic tool for ALV-K and provides a good tool for further research into the molecular mechanisms of interaction between ALV-K env protein and the host cell receptor.
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-017-3563-2