Is MicroRNA-127 a Novel Biomarker for Acute Pancreatitis with Lung Injury?

Background and Aims. The aim of this study was to determine the expression of microRNA-127 (miR-127) in both rat models and patients of acute pancreatitis (AP) with lung injury (LI). Methods. Rats were administrated with retrograde cholangiopancreatography injection of 0.5% or 3.5% sodium taurochola...

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Bibliographic Details
Published in:Disease markers 2017-01, Vol.2017 (2017), p.1-10
Main Authors: Jiang, Kun, Guo, Jia, Yang, Xiaonan, Xia, Qing, Du, Chen, Ma, Yun, Jin, Tao, Luo, Ruijie, Zhang, Xiaoxin, Chen, Weiwei, Deng, Lihui, Shi, Na, Lin, Ziqi
Format: Article
Language:English
Subjects:
Acute Disease
Animal models
Animal tissues
Animals
Biomarkers
Disease Models, Animal
Ethylenediaminetetraacetic acid
Genetic Markers - genetics
Humans
Lung diseases
Lung Injury - etiology
Lung Injury - genetics
Lungs
Male
Medical research
Medicine, Experimental
MicroRNA
MicroRNAs
MicroRNAs - genetics
miRNA
Pancreatitis
Pancreatitis - complications
Pancreatitis - genetics
Patients
Pilot Projects
Polymerase chain reaction
Prospective Studies
Rats
Reverse transcription
Ribonucleic acid
RNA
Rodents
Sodium
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Summary:Background and Aims. The aim of this study was to determine the expression of microRNA-127 (miR-127) in both rat models and patients of acute pancreatitis (AP) with lung injury (LI). Methods. Rats were administrated with retrograde cholangiopancreatography injection of 0.5% or 3.5% sodium taurocholate to induce AP with mild or severe LI and were sacrificed at 6, 12, and 24 h. Rats from the control group received a laparotomy only. Plasma from a prospective cohort of AP patients was collected. The levels of miR-127 in the tissues and plasma were detected using quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Results. The upregulation of miR-127 in the lungs of rats was detected in the groups of AP with severe LI at 6 h and 24 h, whereas it was scarcely detectable in plasma. In the pilot study that included 18 AP patients and 5 healthy volunteers, the plasma miR-127 level was significantly downregulated in AP patients with respiratory failure compared with the healthy volunteers (P=0.014) and those without respiratory failure (P=0.043). Conclusion. miR-127 might serve as a potential marker for the identification of AP with LI.
ISSN:0278-0240
1875-8630
DOI:10.1155/2017/1204295