Gas Chromatography Mass Spectrometry Profiling in Methanolic and Ethyl-acetate Root and Stem Extract of Corbichonia decumbens (Forssk.) Exell from Thar Desert of Rajasthan, India

• Published in: Pharmacognosy research 2017-12, Vol.9 (Suppl 1), p.S48-S52
• Main Authors: Arora, Sunita, Saini, Manju
• Format: Article
• Language: English
• Subjects: Biological activity; Botany; Cancer; Carbohydrates; Chromatography; Flavonoids; Herbal medicine; Mass spectrometry; Molecular weight; Original; Phytochemicals; Plant sciences; Retention; Scientific imaging; Solvents; Ulcers
• ISSN: 0976-4836; 0974-8490; 0974-8490
• DOI: 10.4103/pr.pr_62_17

(Forssk.) Exell ( ), recently has moved to Lophiocarpaceae as per angiospermic plant group (APG) III system, is an annual or short-lived, dwarf, glabrous subshrub, prefers to grow on rocky places and on sand-stones in dry, hot areas of Rajasthan. This is the potential plant with medicinal properties. Vegetative organs under study show antioxidant, anti-inflammatory, antiulcer, antimicrobial, and antinociception activity. This study was carried out to identify the phytoconstituents present in the methanolic and ethyl-acetate extract of root and stem of by GC-MS analysis. Powdered test samples were sequentially extracted with methanol and ethyl-acetate. The compounds obtained as a result of GC-MS screening were identified on the basis of their retention time, peak area and compared with that of literature available and by interpretation of mass spectra. GC-MS analysis of a methanolic extract of root detected mome-inositol (49.53%), guanosine (20.91%), and cis-vaccenic acid (9.25%). While ethyl-acetate extract of root analyzed pentadecanoic acid (17.91%), octadecanoic acid (15.01%) and cis-vaccenic acid (12.04%). Methanolic extract of stem detected mome-inositol (75.47%), pentadecanoic acid (6.04%), and 7-tetradecenal, (Z) (4.54%) while ethyl-acetate extract of stem revealed the presence of 1-heptacosanol (17.35%), hexadecanoic acid (17.17%), and octadecanal (12.64%). The results suggest that (Forssk.) Exell is a plant of potential medicinal value, yielding various bioactive compounds that confirm the application of this plant as a plant-based drug in pharmacy-industry. Extraction is the most important step in the analysis of bioactive compounds present in botanical preparations. The strength of solvent plays a key role in this process, methanol as well as ethyl-acetate showed better response as far as extraction potency is concerned. Gas chromatography mass spectrometry analysis is highly reliable, and the interpretations of the results are of high-quality. This tool is in particular useful for confirming of the presence of bioactive-substances. The results suggest that (Forssk.) Exell can be used for drug formulations against some major disorders, i.e., cancer, ulcer, tuberculosis, arthritis, etc. GC-MS: Gas Chromatography-Mass Spectrometry, kPa: Kilopascal, RT: Retention time, MF: Molecular formula, MW: Molecular weight.