Quantitative analysis of a novel antimicrobial peptide in rat plasma by ultra performance liquid chromatography-tandem mass spectrometry

• Published in: Journal of pharmaceutical analysis 2011-08, Vol.1 (3), p.191-196
• Main Authors: Zhang, Ruo-Wen, Liu, Wen-Tao, Geng, Lu-Lu, Chen, Xiao-Hui, Bi, Kai-Shun
• Format: Article
• Language: English
• Subjects: Antimicrobial peptide; Pharmacokinetic; Phylloseptin; Ultra performance liquid chromatography–tandem mass spectrometry; 串联质谱; 大鼠; 定量分析; 抗菌肽; 药代动力学; 蛋白质沉淀; 血浆; 超高效液相色谱
• ISSN: 2095-1779; 2214-0883
• DOI: 10.1016/j.jpha.2011.04.001

We described the first results of a quantitative ultra performance liquid chromatographytandem mass spectrometry method for a novel antimicrobial peptide （phylloseptin, PSN-1）. Chromatographic separation was accomplished on a Waters bridged ethyl hybrid （BEH） C18 （50mm× 2.1 mm, 1.7 μm） column with acetonitrile-water （25：75, v/v） as isocratic mobile phase. Mass spectrometry detection was performed in the positive electrospray ionization mode and by monitoring of the transitions at m/z 679.6/120, 509.6/120 （PSN-1） and m/z 340.7/165 （Thymopentin, IS）. Protein precipitation was investigated and the recovery was satisfactory （above 82%）. The method was shown to be reproducible and reliable with intra-day precision below 5.3%, inter-day precision below 14.2%, and linear range from 0.02 to 2 lag/mL with r〉0.994. The method was successfully applied to a pharmacokinetic study of PSN-1 in rats after intravenous administration.