Liquid chromatography-tandem mass spectrometry method for the estimation of adefovir in human plasma： Application to a pharmacokinetic study

• Published in: Journal of pharmaceutical analysis 2015-06, Vol.5 (3), p.190-199
• Main Authors: Goswami, Dipanjan, Gurule, Sanjay, Saha, Arabinda, Vats, Poonam, Khuroo, Arshad, Monif, Tausif
• Format: Article
• Language: English
• Subjects: Adefovir; Liquid chromatography–tandem mass spectrometry; Original; Pharmacokinetic study; Solid phase extraction; 人血浆; 估计; 基体效应; 应用; 液相色谱-串联质谱法; 药代动力学; 质谱检测; 阿德福韦酯
• ISSN: 2095-1779; 2214-0883
• DOI: 10.1016/j.jpha.2014.08.002

An analytical method based on solid phase extraction was developed and validated for analysis of adefovir in human plasma. Adefovir-d4 was used as an internal standard and Synergi MAX RP80A （150rnmx4.6mm. 41am） column provided the desired chromatographic separation of compounds followed by detection with mass spectrometry. The method used simple isocratic chromato- graphic condition and mass spectrometric detection in the positive ionization mode. The calibration cuives were linear over the range of 0.50-42.47 ng/mL with the lower limit of quantitation validated at 0.50 ng/mL. Matrix effect was assessed by post-column infusion experiment to monitor phospholipids and post- extraction addition experiment was performed. The degree of matrix effect for adefovir was determined as 7.5% and ion-enhancement in five different lots of human plasma was 7.1% and had no impact on study samples analysis with 4.5 rain run time. The intra- and inter-day precision values were within 7.7% and 7.8%, respectively, for adefovir at the lower limit of quantification level. Validated bioanalytical method was successfully applied to clinical sample analysis.