Non-covalent binding analysis of sulfamethoxazole to human serum albumin： Fluorescence spectroscopy, UV-vis, FT-IR, voltammetric and molecular modeling

• Published in: Journal of pharmaceutical analysis 2015-06, Vol.5 (3), p.143-152
• Main Authors: Naik, Praveen N., Nandibewoor, Sharanappa T., Chimatadar, Shivamurthi A.
• Format: Article
• Language: English
• Subjects: Fluorescence quenching study; Human serum albumin; Original; Static mechanism; Sulfamethoxazole; 人血清白蛋白; 分子模拟技术; 发射光谱分析; 电化学; 磺胺甲基异恶唑; 磺胺甲恶唑; 红外光谱; 荧光光谱
• ISSN: 2095-1779; 2214-0883
• DOI: 10.1016/j.jpha.2015.01.003

This study was designed to examine the interaction of sulfamethoxazole （SMZ） with human serum albumin（HSA）. Spectroscopic analysis of the emission quenching at different temperatures revealed that the quenching mechanism of human serum albumin by SMZ was static mechanism. The binding constant values for the SMZ-HSA system were obtained to be 22,500 L/mol at 288 K, 15,600 L/mol at 298 K, and 8500 L/mol at 308 K. The distance r between donor and acceptor was evaluated according to the theory of Foster energy transfer. The results of spectroscopic analysis and molecular modeling techniques showed that the conformation of human serum albumin had been changed in the presence of SMZ. The thermodynamic parameters, namely enthalpy change （ΔH^0） - 36.0 kJ/mol, entropy change （ΔS^0） - 41.3 Jim01 K and free energy change （ΔG^0） - 23.7 kJ/ mol, were calculated by using van＇t Hoff equation. The effect of common ions on the binding of SMZ to HSA was tested.