A novel biopsy method to increase yield of subcutaneous abdominal adipose tissue

Collection of abdominal subcutaneous adipose tissue (SAT) for research testing is traditionally performed using punch biopsy or needle aspiration techniques, yielding small amounts of very superficial SAT (100–500 mg). Although liposuction techniques can be used to obtain large amounts of SAT, these...

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Bibliographic Details
Published in:International Journal of Obesity 2015-01, Vol.39 (1), p.183-186
Main Authors: Alderete, T L, Sattler, F R, Sheng, X, Tucci, J, Mittelman, S D, Grant, E G, Goran, M I
Format: Article
Language:English
Subjects:
692/699/2743/393
692/700/139
692/700/565/545
Abdomen
Abdominal wall
Adipose tissue
Adipose tissues
Adult
Biological control systems
Biopsy
Biopsy, Needle
Bleeding
Body fat
Cardiovascular disease
Cutting
Epidemiology
Fascia
Fascia - pathology
Female
Flow cytometry
Gene expression
Health Promotion and Disease Prevention
Histology
Humans
Internal Medicine
Liposuction
Male
Medicine
Medicine & Public Health
Metabolic Diseases
Methods
Obesity
Physiological research
Post-translation
Preventive medicine
Properties
Public Health
Reproducibility of Results
Ribonucleic acid
RNA
Shearing
short-communication
Skin
Subcutaneous Fat, Abdominal - pathology
Suction
Ultrasonic imaging
Ultrasound
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Summary:Collection of abdominal subcutaneous adipose tissue (SAT) for research testing is traditionally performed using punch biopsy or needle aspiration techniques, yielding small amounts of very superficial SAT (100–500 mg). Although liposuction techniques can be used to obtain large amounts of SAT, these approaches can compromise the integrity of the adipose tissue. Therefore, we investigated a novel method using a 6-mm Bergström side-cutting biopsy needle to acquire suitable amounts of intact abdominal SAT for multiple complex studies such as flow cytometry, RNA extraction, ex vivo expression of molecular and post-translational protein mediators, and histology. Fifty biopsies were obtained from 29 participants using a Bergström biopsy needle, applying transient manual suction and shearing large pieces of fat within the inner-cutting trochar. Eighteen of the biopsies were performed under ultrasound guidance, whereby we successfully sampled deep SAT (dSAT) from below Scarpa’s fascia. The average weight of SAT sampled was 1.5±0.4 g. There was no clinically important bleeding or ecchymosis on the abdominal wall and no infection occurred with this procedure. The 6-mm Bergström biopsy needle yielded substantially more SAT than what has been obtained from superficial procedures and, for the first time, allowed sampling of dSAT by a percutaneous approach.
ISSN:0307-0565
1476-5497
DOI:10.1038/ijo.2014.90