Inactivated rotavirus vaccine by parenteral administration induces mucosal immunity in mice

To improve the safety and efficacy of oral rotavirus vaccines, we developed an inactivated rotavirus vaccine (IRV) for parenteral administration. Since it remains unknown whether parenteral vaccination can induce mucosal immunity, we performed a comprehensive assessment of immune responses to IRV in...

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Bibliographic Details
Published in:Scientific reports 2018-01, Vol.8 (1), p.561-11, Article 561
Main Authors: Resch, Theresa K., Wang, Yuhuan, Moon, Sung-Sil, Joyce, Jessica, Li, Song, Prausnitz, Mark, Jiang, Baoming
Format: Article
Language:English
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Aluminum sulfate
Antibodies
Antibody response
Antigens
Dendritic cells
Diarrhea
Disease control
Humanities and Social Sciences
Immune response
Immunization
Immunoglobulin G
Infections
Intestinal obstruction
Lymphocytes
Lymphocytes B
Lymphocytes T
Mucosal immunity
multidisciplinary
Polymers
Rotavirus
Science
Science (multidisciplinary)
Spleen
Vaccines
Viruses
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Summary:To improve the safety and efficacy of oral rotavirus vaccines, we developed an inactivated rotavirus vaccine (IRV) for parenteral administration. Since it remains unknown whether parenteral vaccination can induce mucosal immunity, we performed a comprehensive assessment of immune responses to IRV in mice with an adjuvant-free dissolving polymer MN patch or by alum-adjuvanted IM injection. We demonstrated that IRV induced the expression of the gut homing receptor LPAM-1 on T and B cells in spleen and mLN of vaccinated mice. MN patch IRV vaccination induced a slight Th1 phenotype while IM vaccination induced a balanced Th1/Th2 phenotype. In addition, a dose-sparing effect was seen for rotavirus-specific serum IgG and neutralizing activity for both vaccination routes. Our study is the first to show that parenterally administered IRV can induce mucosal immunity in the gut, in addition to strong serum antibody response, and is a promising candidate vaccine in achieving global immunization against rotavirus.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-017-18973-9