Cultivation of Peptidiphaga gingivicola from subgingival plaque: The first representative of a novel genus of Actinomycetaceae

• Published in: Molecular oral microbiology 2018-02, Vol.33 (1), p.105-110
• Main Authors: Beall, C.J., Mokrzan, E.M., Griffen, A.L., Leys, E.J.
• Format: Article
• Language: English
• Subjects: actinobacteria; Actinomycetaceae; Actinomycetaceae - classification; Actinomycetaceae - genetics; Actinomycetaceae - growth & development; Actinomycetaceae - isolation & purification; Alanine; Arginine; Bacteria; Bacterial Typing Techniques; Base Composition; Carbohydrates; Catalase; Conserved sequence; Cultivation; Dental plaque; Dental Plaque - microbiology; DNA, Bacterial - genetics; DNA, Ribosomal - genetics; Esculin; Fatty acids; Fatty Acids - analysis; genome; Genomes; Glycine; Histidine; Humans; Indoles; Leucine; New genera; New species; Nitrate reduction; Periodontal disease; Periodontal Diseases - microbiology; Periodontics; Phenotype; Phylogenetics; Phylogeny; Proline; Proteins; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sequence Analysis, DNA; Serine; Species Specificity; Tyrosine; Urease; Whole Genome Sequencing
• ISSN: 2041-1006; 2041-1014
• DOI: 10.1111/omi.12205

Summary A novel bacterium was isolated from the subgingival plaque of a patient with periodontal disease. Bacterial strain BA112T is a facultative Gram‐positive coccus. It metabolizes alanine, arginine, glycine, histidine, leucine, proline, serine and tyrosine, but does not appear to use carbohydrates. Urease, esculin, indole, catalase and nitrate reduction tests were all negative. Major cellular fatty acids were C18:0, C12:0, C16:0, C18:1 w9c and C20:0. The genome was sequenced and is 2.4 Mbp in length and has 64% GC content. Based on phylogenetics of the 16S rRNA sequence and concatenated alignments of 37 conserved proteins, BA112T belongs to the family Actinomycetaceae but is located on a branch of the tree without currently named members. Based on our phenotypic and phylogenetic studies, we propose that BA112T is the first known representative of a new genus, for which the name Peptidiphaga gingivicola gen. nov., sp. nov. is proposed. The type strain is BA112T.