Abiotic synthesis of purine and pyrimidine ribonucleosides in aqueous microdroplets

Aqueous microdroplets (

Saved in:
Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2018-01, Vol.115 (1), p.36-40
Main Authors: Nam, Inho, Nam, Hong Gil, Zare, Richard N.
Format: Article
Language:English
Subjects:
Adenine
Adenosine
Aqueous solutions
Base pairs
Biological Sciences
Catalysis
Catalysts
Cytosine
D-Ribose
Flight time
Guanine
Guanosine
Hypoxanthine
Magnesium
Mass spectra
Mass spectrometry
Phosphoric acid
Physical Sciences
Pressure
Ribonucleic acid
Ribose
RNA
Uracil
Uridine
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c509t-ac0ddb83facafec46d5d674858bd6ca6b7bf9dcf5a5df3f3fe6c27cd33083c703
cites cdi_FETCH-LOGICAL-c509t-ac0ddb83facafec46d5d674858bd6ca6b7bf9dcf5a5df3f3fe6c27cd33083c703
container_end_page 40
container_issue 1
container_start_page 36
container_title Proceedings of the National Academy of Sciences - PNAS
container_volume 115
creator Nam, Inho
Nam, Hong Gil
Zare, Richard N.
description Aqueous microdroplets (
doi_str_mv 10.1073/pnas.1718559115
format article
fullrecord <record><control><sourceid>jstor_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5776833</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>26506255</jstor_id><sourcerecordid>26506255</sourcerecordid><originalsourceid>FETCH-LOGICAL-c509t-ac0ddb83facafec46d5d674858bd6ca6b7bf9dcf5a5df3f3fe6c27cd33083c703</originalsourceid><addsrcrecordid>eNpdkc1r3DAQxUVpabZpzz21GHrpxcnI8ujjUgihXxDIIe1ZyJLcaPFKrmQX9r-vlk2TJugwiPnNY948Qt5SOKMg2PkcTTmjgkpERSk-IxsKira8V_CcbAA60cq-60_Iq1K2AKBQwkty0qkOETrckJuLIaQl2Kbs43LrSyhNGpt5zSH6xkTXzPscdsEdvjkMKa528qkE50sTYmN-rz6tpdkFm5PLaZ78Ul6TF6OZin9zV0_Jzy-ff1x-a6-uv36_vLhqLYJaWmPBuUGy0Vgzettzh46LXqIcHLeGD2IYlbMjGnQjq89z2wnrGAPJrAB2Sj4dded12HlnfVyymfRcFzZ5r5MJ-nEnhlv9K_3RKASXjFWBj3cCOVUjZdG7UKyfJhMPrjRVQoquB8SKfniCbtOaY7VXKUVR1ov3lTo_UvUapWQ_3i9DQR8C04fA9ENgdeL9_x7u-X8JVeDdEdiWJeWHPkfgFWF_AXNGn0E</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1991584904</pqid></control><display><type>article</type><title>Abiotic synthesis of purine and pyrimidine ribonucleosides in aqueous microdroplets</title><source>JSTOR Archival Journals and Primary Sources Collection</source><source>PubMed Central</source><creator>Nam, Inho ; Nam, Hong Gil ; Zare, Richard N.</creator><creatorcontrib>Nam, Inho ; Nam, Hong Gil ; Zare, Richard N.</creatorcontrib><description>Aqueous microdroplets (&lt;1.3 μm in diameter on average) containing 15 mM D-ribose, 15 mM phosphoric acid, and 5 mM of a nucleobase (uracil, adenine, cytosine, or hypoxanthine) are electrosprayed from a capillary at +5 kV into a mass spectrometer at room temperature and 1 atm pressure with 3 mM divalent magnesium ion (Mg2+) as a catalyst. Mass spectra show the formation of ribonucleosides that comprise a four-letter alphabet of RNA with a yield of 2.5% of uridine (U), 2.5% of adenosine (A), 0.7% of cytidine (C), and 1.7% of inosine (I) during the flight time of ∼50 μs. In the case of uridine, no catalyst is required. An aqueous solution containing guanine cannot be generated under the same conditions given the extreme insolubility of guanine in water. However, inosine can base pair with cytidine and thus substitute for guanosine. Thus, a full set of ribonucleosides to generate the purine–pyrimidine base pairs A-U and I-C are spontaneously generated in aqueous microdroplets under similar mild conditions.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.1718559115</identifier><identifier>PMID: 29255025</identifier><language>eng</language><publisher>United States: National Academy of Sciences</publisher><subject>Adenine ; Adenosine ; Aqueous solutions ; Base pairs ; Biological Sciences ; Catalysis ; Catalysts ; Cytosine ; D-Ribose ; Flight time ; Guanine ; Guanosine ; Hypoxanthine ; Magnesium ; Mass spectra ; Mass spectrometry ; Phosphoric acid ; Physical Sciences ; Pressure ; Ribonucleic acid ; Ribose ; RNA ; Uracil ; Uridine</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 2018-01, Vol.115 (1), p.36-40</ispartof><rights>Volumes 1–89 and 106–114, copyright as a collective work only; author(s) retains copyright to individual articles</rights><rights>Copyright © 2017 the Author(s). Published by PNAS.</rights><rights>Copyright National Academy of Sciences Jan 2, 2018</rights><rights>Copyright © 2017 the Author(s). Published by PNAS. 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c509t-ac0ddb83facafec46d5d674858bd6ca6b7bf9dcf5a5df3f3fe6c27cd33083c703</citedby><cites>FETCH-LOGICAL-c509t-ac0ddb83facafec46d5d674858bd6ca6b7bf9dcf5a5df3f3fe6c27cd33083c703</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/26506255$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/26506255$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793,58238,58471</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29255025$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nam, Inho</creatorcontrib><creatorcontrib>Nam, Hong Gil</creatorcontrib><creatorcontrib>Zare, Richard N.</creatorcontrib><title>Abiotic synthesis of purine and pyrimidine ribonucleosides in aqueous microdroplets</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Aqueous microdroplets (&lt;1.3 μm in diameter on average) containing 15 mM D-ribose, 15 mM phosphoric acid, and 5 mM of a nucleobase (uracil, adenine, cytosine, or hypoxanthine) are electrosprayed from a capillary at +5 kV into a mass spectrometer at room temperature and 1 atm pressure with 3 mM divalent magnesium ion (Mg2+) as a catalyst. Mass spectra show the formation of ribonucleosides that comprise a four-letter alphabet of RNA with a yield of 2.5% of uridine (U), 2.5% of adenosine (A), 0.7% of cytidine (C), and 1.7% of inosine (I) during the flight time of ∼50 μs. In the case of uridine, no catalyst is required. An aqueous solution containing guanine cannot be generated under the same conditions given the extreme insolubility of guanine in water. However, inosine can base pair with cytidine and thus substitute for guanosine. Thus, a full set of ribonucleosides to generate the purine–pyrimidine base pairs A-U and I-C are spontaneously generated in aqueous microdroplets under similar mild conditions.</description><subject>Adenine</subject><subject>Adenosine</subject><subject>Aqueous solutions</subject><subject>Base pairs</subject><subject>Biological Sciences</subject><subject>Catalysis</subject><subject>Catalysts</subject><subject>Cytosine</subject><subject>D-Ribose</subject><subject>Flight time</subject><subject>Guanine</subject><subject>Guanosine</subject><subject>Hypoxanthine</subject><subject>Magnesium</subject><subject>Mass spectra</subject><subject>Mass spectrometry</subject><subject>Phosphoric acid</subject><subject>Physical Sciences</subject><subject>Pressure</subject><subject>Ribonucleic acid</subject><subject>Ribose</subject><subject>RNA</subject><subject>Uracil</subject><subject>Uridine</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNpdkc1r3DAQxUVpabZpzz21GHrpxcnI8ujjUgihXxDIIe1ZyJLcaPFKrmQX9r-vlk2TJugwiPnNY948Qt5SOKMg2PkcTTmjgkpERSk-IxsKira8V_CcbAA60cq-60_Iq1K2AKBQwkty0qkOETrckJuLIaQl2Kbs43LrSyhNGpt5zSH6xkTXzPscdsEdvjkMKa528qkE50sTYmN-rz6tpdkFm5PLaZ78Ul6TF6OZin9zV0_Jzy-ff1x-a6-uv36_vLhqLYJaWmPBuUGy0Vgzettzh46LXqIcHLeGD2IYlbMjGnQjq89z2wnrGAPJrAB2Sj4dded12HlnfVyymfRcFzZ5r5MJ-nEnhlv9K_3RKASXjFWBj3cCOVUjZdG7UKyfJhMPrjRVQoquB8SKfniCbtOaY7VXKUVR1ov3lTo_UvUapWQ_3i9DQR8C04fA9ENgdeL9_x7u-X8JVeDdEdiWJeWHPkfgFWF_AXNGn0E</recordid><startdate>20180102</startdate><enddate>20180102</enddate><creator>Nam, Inho</creator><creator>Nam, Hong Gil</creator><creator>Zare, Richard N.</creator><general>National Academy of Sciences</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20180102</creationdate><title>Abiotic synthesis of purine and pyrimidine ribonucleosides in aqueous microdroplets</title><author>Nam, Inho ; Nam, Hong Gil ; Zare, Richard N.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c509t-ac0ddb83facafec46d5d674858bd6ca6b7bf9dcf5a5df3f3fe6c27cd33083c703</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Adenine</topic><topic>Adenosine</topic><topic>Aqueous solutions</topic><topic>Base pairs</topic><topic>Biological Sciences</topic><topic>Catalysis</topic><topic>Catalysts</topic><topic>Cytosine</topic><topic>D-Ribose</topic><topic>Flight time</topic><topic>Guanine</topic><topic>Guanosine</topic><topic>Hypoxanthine</topic><topic>Magnesium</topic><topic>Mass spectra</topic><topic>Mass spectrometry</topic><topic>Phosphoric acid</topic><topic>Physical Sciences</topic><topic>Pressure</topic><topic>Ribonucleic acid</topic><topic>Ribose</topic><topic>RNA</topic><topic>Uracil</topic><topic>Uridine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nam, Inho</creatorcontrib><creatorcontrib>Nam, Hong Gil</creatorcontrib><creatorcontrib>Zare, Richard N.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium &amp; Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nam, Inho</au><au>Nam, Hong Gil</au><au>Zare, Richard N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Abiotic synthesis of purine and pyrimidine ribonucleosides in aqueous microdroplets</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>2018-01-02</date><risdate>2018</risdate><volume>115</volume><issue>1</issue><spage>36</spage><epage>40</epage><pages>36-40</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>Aqueous microdroplets (&lt;1.3 μm in diameter on average) containing 15 mM D-ribose, 15 mM phosphoric acid, and 5 mM of a nucleobase (uracil, adenine, cytosine, or hypoxanthine) are electrosprayed from a capillary at +5 kV into a mass spectrometer at room temperature and 1 atm pressure with 3 mM divalent magnesium ion (Mg2+) as a catalyst. Mass spectra show the formation of ribonucleosides that comprise a four-letter alphabet of RNA with a yield of 2.5% of uridine (U), 2.5% of adenosine (A), 0.7% of cytidine (C), and 1.7% of inosine (I) during the flight time of ∼50 μs. In the case of uridine, no catalyst is required. An aqueous solution containing guanine cannot be generated under the same conditions given the extreme insolubility of guanine in water. However, inosine can base pair with cytidine and thus substitute for guanosine. Thus, a full set of ribonucleosides to generate the purine–pyrimidine base pairs A-U and I-C are spontaneously generated in aqueous microdroplets under similar mild conditions.</abstract><cop>United States</cop><pub>National Academy of Sciences</pub><pmid>29255025</pmid><doi>10.1073/pnas.1718559115</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0027-8424
ispartof Proceedings of the National Academy of Sciences - PNAS, 2018-01, Vol.115 (1), p.36-40
issn 0027-8424
1091-6490
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5776833
source JSTOR Archival Journals and Primary Sources Collection; PubMed Central
subjects Adenine
Adenosine
Aqueous solutions
Base pairs
Biological Sciences
Catalysis
Catalysts
Cytosine
D-Ribose
Flight time
Guanine
Guanosine
Hypoxanthine
Magnesium
Mass spectra
Mass spectrometry
Phosphoric acid
Physical Sciences
Pressure
Ribonucleic acid
Ribose
RNA
Uracil
Uridine
title Abiotic synthesis of purine and pyrimidine ribonucleosides in aqueous microdroplets
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-08T04%3A54%3A29IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Abiotic%20synthesis%20of%20purine%20and%20pyrimidine%20ribonucleosides%20in%20aqueous%20microdroplets&rft.jtitle=Proceedings%20of%20the%20National%20Academy%20of%20Sciences%20-%20PNAS&rft.au=Nam,%20Inho&rft.date=2018-01-02&rft.volume=115&rft.issue=1&rft.spage=36&rft.epage=40&rft.pages=36-40&rft.issn=0027-8424&rft.eissn=1091-6490&rft_id=info:doi/10.1073/pnas.1718559115&rft_dat=%3Cjstor_pubme%3E26506255%3C/jstor_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c509t-ac0ddb83facafec46d5d674858bd6ca6b7bf9dcf5a5df3f3fe6c27cd33083c703%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1991584904&rft_id=info:pmid/29255025&rft_jstor_id=26506255&rfr_iscdi=true