Neural induction by the node and placode induction by head mesoderm share an initial state resembling neural plate border and ES cells

Around the time of gastrulation in higher vertebrate embryos, inductive interactions direct cells to form central nervous system (neural plate) or sensory placodes. Grafts of different tissues into the periphery of a chicken embryo elicit different responses: Hensen’s node induces a neural plate whe...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2018-01, Vol.115 (2), p.355-360
Main Authors: Trevers, Katherine E., Prajapati, Ravindra S., Hintze, Mark, Stower, Matthew J., Strobl, Anna C., Tambalo, Monica, Ranganathan, Ramya, Moncaut, Natalia, Khan, Mohsin A. F., Stern, Claudio D., Streit, Andrea
Format: Article
Language:English
Subjects:
Animals
Biological Sciences
Central nervous system
Central Nervous System - embryology
Central Nervous System - metabolism
Chick Embryo
Embryo cells
Embryonic Induction
Embryonic Stem Cells - metabolism
Embryos
Explants
Gastrula - embryology
Gastrula - metabolism
Gastrulation
Gene expression
Gene Expression Profiling - methods
Gene Expression Regulation, Developmental
Genes
Grafts
Hensen's node
In Situ Hybridization
Mesoderm
Mesoderm - embryology
Mesoderm - metabolism
Neural plate
Neural Plate - embryology
Neural Plate - metabolism
Skin & tissue grafts
Stem cells
Tissues
Transplantation
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Summary:Around the time of gastrulation in higher vertebrate embryos, inductive interactions direct cells to form central nervous system (neural plate) or sensory placodes. Grafts of different tissues into the periphery of a chicken embryo elicit different responses: Hensen’s node induces a neural plate whereas the head mesoderm induces placodes. How different are these processes? Transcriptome analysis in time course reveals that both processes start by induction of a common set of genes, which later diverge. These genes are remarkably similar to those induced by an extraembryonic tissue, the hypoblast, and are normally expressed in the pregastrulation stage epiblast. Explants of this epiblast grown in the absence of further signals develop as neural plate border derivatives and eventually express lens markers. We designate this state as “preborder”; its transcriptome resembles embryonic stem cells. Finally, using sequential transplantation experiments, we show that the node, head mesoderm, and hypoblast are interchangeable to begin any of these inductions while the final outcome depends on the tissue emitting the later signals.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.1719674115