Gene expression analyses associated with malignant phenotypes of metastatic sub-clones derived from a mouse oral squamous cell carcinoma Sq-1979 cell line

To elucidate the genetic events that occur during the development of OSCC, the present study established a model of oral malignancy using a mouse oral squamous cell carcinoma (OSCC) Sq-1979 cell line. Sq-1979 cells were implanted into syngeneic C3H mice. Subsequently, 233 cells and metastatic sub-cl...

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Bibliographic Details
Published in:Oncology letters 2018-03, Vol.15 (3), p.3350-3356
Main Authors: Adachi, Mitsutaka, Mizuno-Kamiya, Masako, Takayama, Eiji, Kawaki, Harumi, Inagaki, Toshihiro, Sumi, Shigeki, Motohashi, Masayuki, Muramatsu, Yasunori, Sumitomo, Shin-Ichiro, Shikimori, Michio, Yamazaki, Yutaka, Kondoh, Nobuo
Format: Article
Language:English
Subjects:
Animals
Binding proteins
Cancer metastasis
Cancer research
Cancer staging
Cloning
Development and progression
Diagnosis
Experiments
Gene expression
Genetic aspects
Genetic markers
Health aspects
Lymphatic system
Medical prognosis
Metastasis
Mouth cancer
Oral cancer
Penicillin
Physiological aspects
Proteins
Squamous cell carcinoma
Standard deviation
Tumors
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Summary:To elucidate the genetic events that occur during the development of OSCC, the present study established a model of oral malignancy using a mouse oral squamous cell carcinoma (OSCC) Sq-1979 cell line. Sq-1979 cells were implanted into syngeneic C3H mice. Subsequently, 233 cells and metastatic sub-clones (L cells) from primary OSCC, as well as the metastasized lymph node tissues of Sq-1979-implanted mice were established. Compared with parental Sq-1979 and 233 cells, the majority of L cells exhibited a higher proliferation rate and transplantability, and conferred a lower survival rate on the implanted mice. To investigate the genetic background of L cells, preferentially expressed genes in L cells were identified by cDNA microarray and reverse transcription-polymerase chain reaction analyses. The expression of FYN-binding protein ( ), solute carrier family 16 member 13 ( ), keratin 7, transmembrane portion 173 and mRNAs was significantly elevated in L cells compared with that in Sq1979 and 233 cells. The mRNA expression was also evaluated in human OSCC and leukoplakia (LP) tissues. Among the 5 aforementioned mRNAs, the expression of and was significantly higher in OSCC than in LP tissues. Furthermore, the expression of mRNA was significantly elevated in highly invasive OSCCs, which were classified as grades 3 and 4 by the Yamamoto-Kohama (YK) classification of invasion, compared with those in lower grades (YK-1 and -2). The model proposed in the present study could thus describe essential marker genes for the diagnosis of oral malignancies.
ISSN:1792-1074
1792-1082
DOI:10.3892/ol.2017.7648