Sequencing of FKS Hot Spot 1 from Saprochaete capitata To Search for a Relationship to Reduced Echinocandin Susceptibility

, formerly known as , is an emerging fungal pathogen with low susceptibility to echinocandins. Here, we report the nucleotide sequence of the hot spot 1 region of the gene ( HS1), which codifies for the catalytic subunit of β-1,3-d-glucan synthase, the target of echinocandins. For that purpose, we f...

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Bibliographic Details
Published in:Antimicrobial agents and chemotherapy 2018-02, Vol.62 (2)
Main Authors: Arrieta-Aguirre, Inés, Menéndez-Manjón, Pilar, Cuétara, María Soledad, Fernández de Larrinoa, Iñigo, García-Ruiz, Juan Carlos, Moragues, María Dolores
Format: Article
Language:English
Subjects:
Antifungal Agents
Drug Resistance, Fungal
Echinocandins
Fungal Proteins
Gene Expression Regulation, Fungal
Geotrichum
Glucosyltransferases
Mechanisms of Resistance
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Summary:, formerly known as , is an emerging fungal pathogen with low susceptibility to echinocandins. Here, we report the nucleotide sequence of the hot spot 1 region of the gene ( HS1), which codifies for the catalytic subunit of β-1,3-d-glucan synthase, the target of echinocandins. For that purpose, we first designed degenerated oligonucleotide primers derived from conserved flanking regions of the HS1 segment of 12 different fungal species. Interestingly, analysis of the translated HS1 sequences of 12 isolates of revealed that all of them exhibited the same F-to-L substitution in a position that is highly related to reduced echinocandin susceptibility.
ISSN:0066-4804
1098-6596
DOI:10.1128/AAC.01246-17