Fading of wound-induced volatile release during Populus tremula leaf expansion

• Published in: Journal of plant research 2017-01, Vol.130 (1), p.157-165
• Main Authors: Portillo-Estrada, Miguel, Kazantsev, Taras, Niinemets, Ülo
• Format: Article
• Language: English
• Subjects: Biomedical and Life Sciences; Butadienes - metabolism; Chlorophyll - metabolism; Developmental stages; Emissions; Hemiterpenes - metabolism; Herbivory; Leaves; Life Sciences; Lipoxygenase - metabolism; Methanol; Methanol - metabolism; Organic compounds; Pentanes - metabolism; Photosynthesis - physiology; Plant Biochemistry; Plant Ecology; Plant Leaves - chemistry; Plant Leaves - physiology; Plant Physiology; Plant Sciences; Populus - chemistry; Populus - physiology; Populus tremula; Regular Paper; Stress; Time Factors; VOCs; Volatile organic compounds; Volatile Organic Compounds - metabolism
• ISSN: 0918-9440; 1618-0860
• DOI: 10.1007/s10265-016-0880-6

The release of stress-driven volatiles throughout leaf development has been little studied. Therefore, we subjected poplar leaves during their developmental stage (from 2 days to 2 weeks old) to wounding by a single punch hole, and measured online the wound-induced volatile organic compound emissions. Our study shows that the emission of certain volatile compounds fades with increasing leaf age. Among these compounds we found lipoxygenase products (LOX products), acetaldehyde, methyl benzoate, methyl salicylate, and mono- and sesquiterpenes. In parallel, we studied the fading of constitutive emissions of methanol during leaf maturation, as well as the rise in isoprene constitutive emission during leaf maturation and its relationship to leaf photosynthetic capacity. We found highly significant relationships between leaf chlorophyll content, photosynthetic capacity, and leaf size during leaf ageing. As the level of constitutive defences increases with increasing leaf age, the strength of the volatile signal is expected to be gradually reduced. The higher elicitation of volatile organic compound emissions (especially LOX products) in younger leaves could be an evolutionary defence against herbivory, given that younger leaves are usually more subjected to infestation and herbivory.