Embryonic exposure to ethanol increases the susceptibility of larval zebrafish to chemically induced seizures

Prenatal ethanol exposure is known to cause neurodevelopmental disorders. While high prevalence of epilepsy is observed among the children whose mothers abused alcohol during pregnancy, the results from animal studies are conflicting. Here, we investigated whether embryonic exposure to ethanol can i...

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Bibliographic Details
Published in:Scientific reports 2018-01, Vol.8 (1), p.1845-10, Article 1845
Main Authors: Wang, Keling, Chen, Xiaopan, Liu, Jie, Zou, Li-Ping, Feng, Wenke, Cai, Lu, Wu, Xiaoyang, Chen, Shao-yu
Format: Article
Language:English
Subjects:
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64/116
692/617/375
Animals
Children
Convulsants - pharmacology
Danio rerio
Disease Models, Animal
Embryo, Nonmammalian - drug effects
Epilepsy
Epilepsy - chemically induced
Ethanol
Ethanol - adverse effects
Exposure
Fertilization
Humanities and Social Sciences
Larva - drug effects
Larvae
Latency
multidisciplinary
Neurodevelopmental disorders
Pentylenetetrazole
Pentylenetetrazole - pharmacology
Pregnancy
Prenatal experience
Science
Science (multidisciplinary)
Seizures
Seizures - chemically induced
Zebrafish
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Summary:Prenatal ethanol exposure is known to cause neurodevelopmental disorders. While high prevalence of epilepsy is observed among the children whose mothers abused alcohol during pregnancy, the results from animal studies are conflicting. Here, we investigated whether embryonic exposure to ethanol can increase the susceptibility to pentylenetetrazole (PTZ)-induced seizures in larval zebrafish. Embryos at 3 hours post-fertilization (hpf) were exposed to ethanol at the concentrations ranging from 0.25% to 1% for 21 hours. Control and ethanol-exposed larvae were challenged with PTZ at 7 days post-fertilization (dpf) at the concentrations of 2.5, 5 or 15 mM. The seizure behavior of larvae was recorded and analyzed using EthoVision XT 11. We found that embryonic ethanol exposure increased the percentage of larvae exhibiting typical stage II and III seizure and resulted in a significant reduction in stage I, II and III seizure latency in an ethanol concentration-dependent manner. Embryonic exposure to ethanol also significantly increased the severity of PTZ-induced seizures in larvae, as demonstrated by increased total distance traveled and the duration of mobility. This is the first demonstration that ethanol exposure during early embryonic stage can reduce the threshold for chemically induced seizures and increase the severity of seizure behavior in larval fish.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-018-20288-2