Development and validation of a functional co-dominant SNP marker for the photoperiod thermo-sensitive genic male sterility pms3 (p/tms12-1) gene in rice

The fertility conversion of photoperiod thermo-sensitive genic male sterility (P/TGMS) lines in rice is mostly regulated by the P/TGMS genes in different environmental conditions. A point mutation with G-C on the pms3 (p/tms12-1) gene regulates the pollen fertility of Nongken58S and a large amount o...

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Bibliographic Details
Published in:Breeding Science 2017, Vol.67(5), pp.535-539
Main Authors: Qi, Yongbin, Wang, Linyou, Gui, Junmei, Zhang, Lixia, Liu, Qinglong, Wang, Jianjun
Format: Article
Language:English
Subjects:
Breeding
Cultivars
Environmental conditions
Fertility
functional marker
Genetic resources
Genotype & phenotype
Genotypes
Hybrid systems
Inbreeding
Male sterility
Marker-assisted selection
MAS
Mutation
Oryza
Oryza sativa
photoperiod-thermo-sensitive genic male sterility (P/TGMS)
Point mutation
Pollen
Population genetics
Rice
Seedlings
Seeds
Single-nucleotide polymorphism
SNP
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Summary:The fertility conversion of photoperiod thermo-sensitive genic male sterility (P/TGMS) lines in rice is mostly regulated by the P/TGMS genes in different environmental conditions. A point mutation with G-C on the pms3 (p/tms12-1) gene regulates the pollen fertility of Nongken58S and a large amount of Nongken58S-derived lines. In this study, we developed and designed a functional co-dominant marker according to the SNP loci for the pms3 (p/tms12-1) gene. We can differentiate the SNP loci in pms3 (p/tms12-1) gene from another TGMS lines and inbred cultivars using the dpms3-54 marker. The results showed that 376 bp band was detected in the homozygous genotype for pms3 (p/tms12-1), while 359 bp band was detected in the homozygous genotype for non-pms3 (p/tms12-1), two bands with 376 and 359 bp were detected in the heterozygous genotype. The dpms3-54 marker can be used to test the purity of two-line hybrid rice seeds and to divide each of F1 plant into homozygous and heterozygous genotypes at the seedling stage. Thus, this study provide a useful functional marker to detect pms3 (p/tms12-1) gene in different genetic resources and populations, which can be applied to the development and breeding of P/TGMS lines in two-line hybrid rice system with marker-assisted selection.
ISSN:1344-7610
1347-3735
DOI:10.1270/jsbbs.16138