Novel Application of Cultured Epithelial Autografts (CEA) with Expanded Mesh Skin Grafting Over an Artificial Dermis or Dermal Wound Bed Preparation

Cultured epithelial autografts (CEA) with highly expanded mesh skin grafts were used for extensive adult burns covering more than 30% of the total body surface area. A prospective study on eight patients assessed subjective and objective findings up to a 12-month follow-up. The results of wound heal...

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Bibliographic Details
Published in:International journal of molecular sciences 2018-01, Vol.19 (1), p.57
Main Authors: Akita, Sadanori, Hayashida, Kenji, Yoshimoto, Hiroshi, Fujioka, Masaki, Senju, Chikako, Morooka, Shin, Nishimura, Gozo, Mukae, Nobuhiko, Kobayashi, Kazuo, Anraku, Kuniaki, Murakami, Ryuichi, Hirano, Akiyoshi, Oishi, Masao, Ikenoya, Shintaro, Amano, Nobuyuki, Nakagawa, Hiroshi
Format: Article
Language:English
Subjects:
Aged
Autografts
Autografts - transplantation
Cells, Cultured
Cicatrix - pathology
Dermis
Dermis - pathology
Dermis - transplantation
Epithelial Cells - transplantation
Female
Grafting
Grafts
Humans
Linear Models
Male
Middle Aged
Scars
Skin
Skin & tissue grafts
Skin grafts
Skin preparations
Skin Transplantation
Skin, Artificial
Statistical models
Viscoelasticity
Water loss
Wound Healing
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Summary:Cultured epithelial autografts (CEA) with highly expanded mesh skin grafts were used for extensive adult burns covering more than 30% of the total body surface area. A prospective study on eight patients assessed subjective and objective findings up to a 12-month follow-up. The results of wound healing for over 1:6 mesh plus CEA, gap 1:6 mesh plus CEA, and 1:3 mesh were compared at 3, 6, and 12 months using extensibility, viscoelasticity, color, and transepidermal water loss by a generalized estimating equation (GEE) or generalized linear mixed model (GLMM). No significant differences were observed among the paired treatments at any time point. At 6 and 12 months, over 1:6 mesh plus CEA achieved significantly better expert evaluation scores by the Vancouver and Manchester Scar Scales ( < 0.01). Extended skin grafting plus CEA minimizes donor resources and the quality of scars is equal or similar to that with conventional low extended mesh slit-thickness skin grafting such as 1:3 mesh. A longitudinal analysis of scars may further clarify the molecular changes of scar formation and pathogenesis.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms19010057