Aeginetia indica Decoction Inhibits Hepatitis C Virus Life Cycle

Chronic hepatitis C virus (HCV) infection is still a global epidemic despite the introduction of several highly effective direct-acting antivirals that are tagged with sky-high prices. The present study aimed to identify an herbal decoction that ameliorates HCV infection. Among six herbal decoctions...

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Bibliographic Details
Published in:International journal of molecular sciences 2018-01, Vol.19 (1), p.208
Main Authors: Lin, Cheng-Wei, Lo, Chieh-Wen, Tsai, Chia-Ni, Pan, Ting-Chun, Chen, Pin-Yin, Yu, Ming-Jiun
Format: Article
Language:English
Subjects:
Aeginetia indica
Antiviral agents
Cell Line, Tumor
Cell Survival - drug effects
Chronic infection
Epidemics
Hepacivirus - drug effects
Hepacivirus - metabolism
Hepatitis
Hepatitis C
Hepatocytes
Humans
Infections
Interferon
Internal ribosome entry site
Liver
Orobanchaceae - chemistry
Orobanchaceae - metabolism
Phosphorylation
Phosphorylation - drug effects
Plant Extracts - chemistry
Plant Extracts - pharmacology
Pretreatment
Proteins
Replication
RNA, Viral - metabolism
Serine
Translation
Viral Nonstructural Proteins - metabolism
Virus Replication - drug effects
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Summary:Chronic hepatitis C virus (HCV) infection is still a global epidemic despite the introduction of several highly effective direct-acting antivirals that are tagged with sky-high prices. The present study aimed to identify an herbal decoction that ameliorates HCV infection. Among six herbal decoctions tested, the decoction had the most profound effect on the HCV reporter activity in infected Huh7.5.1 liver cells in a dose- and time-dependent manner. The decoction exerted multiple inhibitory effects on the HCV life cycle. Pretreatment of the cells with the decoction prior to HCV infection reduced the HCV RNA and non-structural protein 3 (NS3) protein levels in the infected cells. The decoction reduced HCV internal ribosome entry site-mediated protein translation activity. It also reduced the HCV RNA level in the infected cells in association with reduced NS5A phosphorylation at serine 235, a predominant phosphorylation event indispensable to HCV replication. Thus, the decoction inhibits HCV infection, translation, and replication. Mechanistically, the decoction probably reduced HCV replication via reducing NS5A phosphorylation at serine 235.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms19010208