Demonstration of ion channel synthesis by isolated squid giant axon provides functional evidence for localized axonal membrane protein translation

• Published in: Scientific reports 2018-02, Vol.8 (1), p.2207-13, Article 2207
• Main Authors: Mathur, Chhavi, Johnson, Kory R., Tong, Brian A., Miranda, Pablo, Srikumar, Deepa, Basilio, Daniel, Latorre, Ramon, Bezanilla, Francisco, Holmgren, Miguel
• Format: Article
• Language: English
• Subjects: 38; 631/337/2019; 631/378/2586; 631/378/87; 64; Animals; Axons; Axons - metabolism; Cells, Cultured; Decapodiformes; Dendrites; Drosophila; Drosophila Proteins - biosynthesis; Drosophila Proteins - genetics; Endoplasmic reticulum; Giant axons; Humanities and Social Sciences; Inactivation; Ion channels; Ion Channels - biosynthesis; Ion Channels - genetics; Kinetics; Membrane proteins; Membranes; mRNA; multidisciplinary; Patch-Clamp Techniques; Potassium currents; Protein Biosynthesis; Protein synthesis; Proteins; Recombinant Proteins - biosynthesis; Recombinant Proteins - genetics; rRNA; Science; Science (multidisciplinary); Signal recognition particle; Translation; Voltage
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-018-20684-8

Local translation of membrane proteins in neuronal subcellular domains like soma, dendrites and axon termini is well-documented. In this study, we isolated the electrical signaling unit of an axon by dissecting giant axons from mature squids ( Dosidicus gigas ). Axoplasm extracted from these axons was found to contain ribosomal RNAs, ~8000 messenger RNA species, many encoding the translation machinery, membrane proteins, translocon and signal recognition particle (SRP) subunits, endomembrane-associated proteins, and unprecedented proportions of SRP RNA (~68% identical to human homolog). While these components support endoplasmic reticulum-dependent protein synthesis, functional assessment of a newly synthesized membrane protein in axolemma of an isolated axon is technically challenging. Ion channels are ideal proteins for this purpose because their functional dynamics can be directly evaluated by applying voltage clamp across the axon membrane. We delivered in vitro transcribed RNA encoding native or Drosophila voltage-activated Shaker K V channel into excised squid giant axons. We found that total K + currents increased in both cases; with added inactivation kinetics on those axons injected with RNA encoding the Shaker channel. These results provide unambiguous evidence that isolated axons can exhibit de novo synthesis, assembly and membrane incorporation of fully functional oligomeric membrane proteins.