The rolB plant oncogene affects multiple signaling protein modules related to hormone signaling and plant defense

• Published in: Scientific reports 2018-02, Vol.8 (1), p.2285-14, Article 2285
• Main Authors: Bulgakov, Victor P., Vereshchagina, Yulia V., Bulgakov, Dmitry V., Veremeichik, Galina N., Shkryl, Yuri N.
• Format: Article
• Language: English
• Subjects: 13/106; 45/29; 45/77; 631/114; 631/449; 631/61; 82/58; 82/81; Agrobacterium - metabolism; Apoptosis; Arabidopsis - chemistry; Arabidopsis - metabolism; Arabidopsis - microbiology; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; beta-Glucosidase - genetics; beta-Glucosidase - metabolism; Bioinformatics; Calcium signalling; Callus; Cell death; Cells, Cultured; DnaK protein; Environmental stress; Gene expression; Gene Expression Profiling; Heat shock proteins; Herbicides; Humanities and Social Sciences; miRNA; multidisciplinary; Oncogenes; Peptidylprolyl isomerase; Plant cells; Plant Cells - chemistry; Plant Cells - metabolism; Plant Diseases - microbiology; Plant Growth Regulators - metabolism; Plant Proteins - analysis; Plant Proteins - genetics; Proteins; Proteome - analysis; Proteomics; Real-Time Polymerase Chain Reaction; Science; Science (multidisciplinary); Signal Transduction
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-018-20694-6

The rolB plant oncogene of Agrobacterium rhizogenes perturbs many biochemical processes in transformed plant cells, thereby causing their neoplastic reprogramming. The oncogene renders the cells more tolerant to environmental stresses and herbicides and inhibits ROS elevation and programmed cell death. In the present work, we performed a proteomic analysis of Arabidopsis thaliana rolB -expressing callus line AtB-2, which represents a line with moderate expression of the oncogene. Our results show that under these conditions rolB greatly perturbs the expression of some chaperone-type proteins such as heat-shock proteins and cyclophilins. Heat-shock proteins of the DnaK subfamily were overexpressed in rolB -transformed calli, whereas the abundance of cyclophilins, members of the closely related single-domain cyclophilin family was decreased. Real-time PCR analysis of corresponding genes confirmed the reliability of proteomics data because gene expression correlated well with the expression of proteins. Bioinformatics analysis indicates that rolB can potentially affect several levels of signaling protein modules, including effector-triggered immunity (via the RPM1-RPS2 signaling module), the miRNA processing machinery, auxin and cytokinin signaling, the calcium signaling system and secondary metabolism.