Cellular basis of angiotensin-(1-7)-induced augmentation of left ventricular functional performance in heart failure

• Published in: International journal of cardiology 2017-06, Vol.236, p.405-412
• Main Authors: Zhang, Xiaowei, Cheng, Heng-Jie, Zhou, Peng, Kitzman, Dalane W, Ferrario, Carlos M, Li, Wei-Min, Cheng, Che Ping
• Format: Article
• Language: English
• Subjects: Angiotensin I - pharmacology; Angiotensin I - therapeutic use; Angiotensin-(1-7); Animals; Calcium transient; Cardiovascular; Cells; Contractility; Heart failure; Heart Failure - drug therapy; Heart Failure - physiopathology; Male; Myocytes, Cardiac - drug effects; Myocytes, Cardiac - physiology; Peptide Fragments - pharmacology; Peptide Fragments - therapeutic use; Pressure-volume relation; Rats; Vasodilator Agents - pharmacology; Vasodilator Agents - therapeutic use; Ventricular Function, Left - drug effects; Ventricular Function, Left - physiology
• ISSN: 0167-5273; 1874-1754
• DOI: 10.1016/j.ijcard.2017.01.071

Abstract Background Angiotensin-(1-7) [Ang-(1-7)] exhibits cardiovascular effects opposite those of angiotensin II (Ang II), thus providing protection against heart disease. However, how Ang-(1-7) imparts cardioprotection is unclear, and its direct cardiac effects are controversial. Whether heart failure (HF) alters cardiac contractile responses to Ang-(1-7) remains undetermined. We tested the hypothesis that in HF, Ang-(1-7) may produce positive modulation on [Ca 2 + ]i regulation, enhancing left ventricular (LV) and myocyte contraction and relaxation via Ang-(1-7) Mas receptor coupled with nitric oxide (NO)/bradykinin (BK)-mediated mechanism. Methods and results We measured LV contractility changes after Ang-(1-7) (650 ng/kg, iv) and compared myocyte functional and [Ca 2 + ]i transient ([Ca 2 + ]iT ) responses to Ang-(1-7) superfusion in 24 normal rats and 34 rats with isoproterenol-induced HF (3 months after 170 mg/kg, s.q. for 2 days). To assess the mechanisms of altered HF responses to Ang-(1-7), subsets of HF myocytes were pretreated to inhibit NO synthase (L-NAME), BK (HOE-140), and Mas receptor (A-779) followed with Ang-(1-7). In normal rats, Ang-(1-7) produced no significant changes in LV and myocyte function. In HF rats, Ang-(1-7) significantly augmented LV contractility and relaxation with increased EES (51%), but decreased τ compared to baseline. Ang-(1-7) also significantly increased myocyte contraction (dL/dtmax , 30%), relaxation (dR/dtmax , 41%), and [Ca 2 + ]iT . L-NAME increased, HOE-140 decreased, and A-779 prevented HF myocyte contractile responses to Ang-(1-7). Conclusions In a rat model of HF, Ang-(1-7) increases [Ca 2 + ]iT , and produces positive inotropic and lusitropic effects in the LV and myocytes. These effects are mediated by the Mas receptor and involve activation of NO/BK pathways.