Protective effects of quercetin glycosides, rutin, and isoquercetrin against 6-hydroxydopamine (6-OHDA)-induced neurotoxicity in rat pheochromocytoma (PC-12) cells

There is increasing evidence that free radicals induced oxidative stress is a major causative agent in the pathogenesis of neurodegenerative diseases, particularly Parkinson’s disease. Quercetin glycosides, namely rutin and isoquercitrin, are flavonoid polyphenol compounds found ubiquitously in frui...

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Bibliographic Details
Published in:International journal of immunopathology and pharmacology 2016-03, Vol.29 (1), p.30-39
Main Authors: Magalingam, Kasthuri Bai, Radhakrishnan, Ammu, Haleagrahara, Nagaraja
Format: Article
Language:English
Subjects:
6-Hydroxydopamine
Animals
Antioxidants
Catalase
Enzymes
Flavonoids
Free radicals
Glutathione peroxidase
Glycosides
Lipid peroxidation
Malondialdehyde
Movement disorders
Neurodegenerative diseases
Neuroprotection
Neuroprotective Agents - pharmacology
Neurotoxicity
Original
Oxidative stress
Oxidopamine - toxicity
Parkinson's disease
PC12 Cells
Quercetin
Quercetin - analogs & derivatives
Quercetin - pharmacology
Rats
Rutin
Rutin - pharmacology
Superoxide dismutase
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Summary:There is increasing evidence that free radicals induced oxidative stress is a major causative agent in the pathogenesis of neurodegenerative diseases, particularly Parkinson’s disease. Quercetin glycosides, namely rutin and isoquercitrin, are flavonoid polyphenol compounds found ubiquitously in fruits and vegetables and have been known to possess antioxidant effects. This study was designed to compare the neuroprotective effects of quercetin glycosides rutin and isoquercitrin in 6-OHDA-induced rat pheochromocytoma (PC-12) cells. The results showed that both rutin and isoquercitrin significantly increased antioxidant enzymes, catalase, superoxide dismutase, glutathione peroxidase, and glutathione level that were attenuated by 6-OHDA in PC-12 cells. There was no significant difference in the activation of glutathione and glutathione peroxidase enzymes between rutin and isoquercitrin. These two glycosides were equally effective in suppressing lipid peroxidation in 6-OHDA-induced PC-12 cells as both compounds suppressed the malondialdehyde generation and prevented cell damage. In conclusion, quercetin glycosides rutin and isoquercetrin are having a significant neuroprotective effect against 6-OHDA toxicity in PC-12 cells.
ISSN:2058-7384
0394-6320
2058-7384
DOI:10.1177/0394632015613039