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The SlCBL10 Calcineurin B-Like Protein Ensures Plant Growth under Salt Stress by Regulating Na+ and Ca2+ Homeostasis1
Tomato CALCINEURIN B-LIKE PROTEIN 10 (SlCBL10) ensures plant growth by regulating proper distribution of Na+ and Ca2+ in the shoot apical meristem and developing organs under salt stress. Characterization of a new tomato ( Solanum lycopersicum ) T-DNA mutant allowed for the isolation of the CALCINEU...
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Published in: | Plant physiology (Bethesda) 2017-12, Vol.176 (2), p.1676-1693 |
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Main Authors: | , , , , , , , , , , , , , , , , , , , |
Format: | Article |
Language: | English |
Online Access: | Get full text |
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Summary: | Tomato CALCINEURIN B-LIKE PROTEIN 10 (SlCBL10) ensures plant growth by regulating proper distribution of Na+ and Ca2+ in the shoot apical meristem and developing organs under salt stress.
Characterization of a new tomato (
Solanum lycopersicum
) T-DNA mutant allowed for the isolation of the
CALCINEURIN B-LIKE PROTEIN 10
(
SlCBL10
) gene whose lack of function was responsible for the severe alterations observed in the shoot apex and reproductive organs under salinity conditions. Physiological studies proved that
SlCBL10
gene is required to maintain a proper low Na
+
/Ca
2+
ratio in growing tissues allowing tomato growth under salt stress. Expression analysis of the main responsible genes for Na
+
compartmentalization (i.e.
Na
+
/H
+
EXCHANGERs
,
SALT OVERLY SENSITIVE
,
HIGH-AFFINITY K+ TRANSPORTER 1;2
,
H
+
-pyrophosphatase AVP1
[
SlAVP1
] and V-ATPase [
SlVHA-A1
]) supported a reduced capacity to accumulate Na
+
in
Slcbl10
mutant leaves, which resulted in a lower uploading of Na
+
from xylem, allowing the toxic ion to reach apex and flowers. Likewise, the tomato
CATION EXCHANGER 1
and
TWO-PORE CHANNEL 1
(
SlTPC1
), key genes for Ca
2+
fluxes to the vacuole, showed abnormal expression in
Slcbl10
plants indicating an impaired Ca
2+
release from vacuole. Additionally, complementation assay revealed that
SlCBL10
is a true ortholog of the Arabidopsis (
Arabidopsis thaliana
)
CBL10
gene, supporting that the essential function of CBL10 is conserved in Arabidopsis and tomato. Together, the findings obtained in this study provide new insights into the function of
SlCBL10
in salt stress tolerance. Thus, it is proposed that SlCBL10 mediates salt tolerance by regulating Na
+
and Ca
2+
fluxes in the vacuole, cooperating with the vacuolar cation channel
SlTPC1
and the two vacuolar H
+
-pumps,
SlAVP1
and
SlVHA-A1
, which in turn are revealed as potential targets of
SlCBL10
. |
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ISSN: | 0032-0889 1532-2548 |
DOI: | 10.1104/pp.17.01605 |