Polygonum aviculare L. extract and quercetin attenuate contraction in airway smooth muscle

• Published in: Scientific reports 2018-02, Vol.8 (1), p.3114-12, Article 3114
• Main Authors: Luo, Xi, Xue, Lu, Xu, Hao, Zhao, Qing-Yang, Wang, Qian, She, Yu-Shan, Zang, Dun-An, Shen, Jinhua, Peng, Yong-Bo, Zhao, Ping, Yu, Meng-Fei, Chen, Weiwei, Ma, Li-Qun, Chen, Shu, Chen, Shanshan, Fu, Xiangning, Hu, Sheng, Nie, Xiaowei, Shen, Chenyou, Zou, Chunbin, Qin, Gangjian, Dai, Jiapei, Ji, Guangju, Su, Yunchao, Hu, Shen, Chen, Jingyu, Liu, Qing-Hua
• Format: Article
• Language: English
• Subjects: 14/19; 631/45/269/1146; 631/80/86/1999; 64/60; 9/74; Acetic acid; Acetylcholine; Acetylcholine - pharmacology; Animals; Aquatic plants; Asthma; Bronchodilators; Calcium - metabolism; Calcium channels; Calcium channels (L-type); Calcium channels (voltage-gated); Calcium Channels, L-Type - metabolism; Chronic obstructive pulmonary disease; Contraction; Ethyl acetate; Humanities and Social Sciences; Humans; Lung - drug effects; Lung - metabolism; Lung diseases; Male; Mice; Mice, Inbred BALB C; multidisciplinary; Muscle contraction; Muscle Contraction - drug effects; Muscle, Smooth - drug effects; Muscle, Smooth - metabolism; Nifedipine; Nifedipine - pharmacology; Obstructive lung disease; Plant Extracts - pharmacology; Polygonum - chemistry; Polygonum aviculare; Quercetin; Quercetin - pharmacology; Respiratory tract; Science; Science (multidisciplinary); Smooth muscle; TRPC Cation Channels - metabolism
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-018-20409-x

Because of the serious side effects of the currently used bronchodilators, new compounds with similar functions must be developed. We screened several herbs and found that Polygonum aviculare L . contains ingredients that inhibit the precontraction of mouse and human airway smooth muscle (ASM). High K + -induced precontraction in ASM was completely inhibited by nifedipine, a selective blocker of L-type voltage-dependent Ca 2+ channels (LVDCCs). However, nifedipine only partially reduced the precontraction induced by acetylcholine chloride (ACH). Additionally, the ACH-induced precontraction was partly reduced by pyrazole-3 (Pyr3), a selective blocker of TRPC3 and stromal interaction molecule (STIM)/Orai channels. These channel-mediated currents were inhibited by the compounds present in P . aviculare extracts, suggesting that this inhibition was mediated by LVDCCs, TRPC3 and/or STIM/Orai channels. Moreover, these channel-mediated currents were inhibited by quercetin, which is present in P . aviculare extracts. Furthermore, quercetin inhibited ACH-induced precontraction in ASM. Overall, our data indicate that the ethyl acetate fraction of P . aviculare and quercetin can inhibit Ca 2+ -permeant LVDCCs, TRPC3 and STIM/Orai channels, which inhibits the precontraction of ASM. These findings suggest that P . aviculare could be used to develop new bronchodilators to treat obstructive lung diseases such as asthma and chronic obstructive pulmonary disease.