High Expression of ITGA3 Promotes Proliferation and Cell Cycle Progression and Indicates Poor Prognosis in Intrahepatic Cholangiocarcinoma

Integrin subunit alpha 3 (ITGA3) interacts with a beta 1 subunit to form a member of the integrin family. Integrins are heterodimeric integral membrane proteins that serve as cell surface adhesion proteins. In this research, we investigated the biological function of this protein in human intrahepat...

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Published in:BioMed research international 2018-01, Vol.2018 (2018), p.1-9
Main Authors: Lu, Sinan, Zhou, Lin, Xie, Haiyang, Zheng, Shusen, Wang, Weilin, Zhou, Dongkai, Zhang, Linshi, Yan, Yingcai, Zhou, Xiaohu, He, Tianyu, Zhang, Lufei, Kong, Yang, Huang, Yu, Zhou, Jiarong
Format: Article
Language:English
Subjects:
Adhesion
Biotechnology
Cancer therapies
Cell culture
Cell cycle
Cell proliferation
Cell surface
Cholangiocarcinoma
Extracellular matrix
Hospitals
Immunohistochemistry
Integral membrane proteins
Integrins
Laboratories
Liver cancer
Lymph nodes
Medical diagnosis
Medical prognosis
Medicine
Melanoma
Membrane proteins
Metastases
Metastasis
MicroRNAs
Patients
Prognosis
Prostate cancer
Proteins
Rodents
Surgery
Surgical outcomes
Tumors
Western blotting
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cited_by cdi_FETCH-LOGICAL-c499t-bc2a1c7bc3fb4047d7e6a3349a66510cea05dddc995b45aede67f414f37104903
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creator Lu, Sinan
Zhou, Lin
Xie, Haiyang
Zheng, Shusen
Wang, Weilin
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Zhang, Linshi
Yan, Yingcai
Zhou, Xiaohu
He, Tianyu
Zhang, Lufei
Kong, Yang
Huang, Yu
Zhou, Jiarong
description Integrin subunit alpha 3 (ITGA3) interacts with a beta 1 subunit to form a member of the integrin family. Integrins are heterodimeric integral membrane proteins that serve as cell surface adhesion proteins. In this research, we investigated the biological function of this protein in human intrahepatic cholangiocarcinoma (ICC) for the first time. Here, using Western blotting and immunohistochemistry assays, we discovered that ITGA3 was overexpressed in ICC cell lines and ICC patients. Moreover, we found ITGA3 expression correlated with several clinicopathological features, including tumor size, lymph node metastasis, and the TNM stage. Patients with high ITGA3 expression underwent a worse prognosis after complete resection compared with patients with low ITGA3 expression in terms of overall survival. Furthermore, we demonstrated that ITGA3 could significantly promote ICC cell proliferation and cell cycle progression in vitro. However, as a classical cell surface adhesion molecule, we found ITGA3 correlated negatively with the migration and invasion of ICC cell lines, which differs from other malignant tumors. Generally, these findings suggest that ITGA3 may play a role as a potential oncogene in ICC and suppression of ITGA3 expression may establish a novel target for guiding the therapy of ICC patients.
doi_str_mv 10.1155/2018/2352139
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Integrins are heterodimeric integral membrane proteins that serve as cell surface adhesion proteins. In this research, we investigated the biological function of this protein in human intrahepatic cholangiocarcinoma (ICC) for the first time. Here, using Western blotting and immunohistochemistry assays, we discovered that ITGA3 was overexpressed in ICC cell lines and ICC patients. Moreover, we found ITGA3 expression correlated with several clinicopathological features, including tumor size, lymph node metastasis, and the TNM stage. Patients with high ITGA3 expression underwent a worse prognosis after complete resection compared with patients with low ITGA3 expression in terms of overall survival. Furthermore, we demonstrated that ITGA3 could significantly promote ICC cell proliferation and cell cycle progression in vitro. However, as a classical cell surface adhesion molecule, we found ITGA3 correlated negatively with the migration and invasion of ICC cell lines, which differs from other malignant tumors. Generally, these findings suggest that ITGA3 may play a role as a potential oncogene in ICC and suppression of ITGA3 expression may establish a novel target for guiding the therapy of ICC patients.</description><identifier>ISSN: 2314-6133</identifier><identifier>EISSN: 2314-6141</identifier><identifier>DOI: 10.1155/2018/2352139</identifier><identifier>PMID: 29511671</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Adhesion ; Biotechnology ; Cancer therapies ; Cell culture ; Cell cycle ; Cell proliferation ; Cell surface ; Cholangiocarcinoma ; Extracellular matrix ; Hospitals ; Immunohistochemistry ; Integral membrane proteins ; Integrins ; Laboratories ; Liver cancer ; Lymph nodes ; Medical diagnosis ; Medical prognosis ; Medicine ; Melanoma ; Membrane proteins ; Metastases ; Metastasis ; MicroRNAs ; Patients ; Prognosis ; Prostate cancer ; Proteins ; Rodents ; Surgery ; Surgical outcomes ; Tumors ; Western blotting</subject><ispartof>BioMed research international, 2018-01, Vol.2018 (2018), p.1-9</ispartof><rights>Copyright © 2018 Yu Huang et al.</rights><rights>COPYRIGHT 2018 John Wiley &amp; Sons, Inc.</rights><rights>Copyright © 2018 Yu Huang et al.; This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright © 2018 Yu Huang et al. 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c499t-bc2a1c7bc3fb4047d7e6a3349a66510cea05dddc995b45aede67f414f37104903</citedby><cites>FETCH-LOGICAL-c499t-bc2a1c7bc3fb4047d7e6a3349a66510cea05dddc995b45aede67f414f37104903</cites><orcidid>0000-0003-1459-8261 ; 0000-0001-9432-2649 ; 0000-0002-6270-0737</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2002928552/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2002928552?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,776,780,881,25731,27901,27902,36989,36990,44566,74869</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29511671$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Aishima, Shinichi</contributor><creatorcontrib>Lu, Sinan</creatorcontrib><creatorcontrib>Zhou, Lin</creatorcontrib><creatorcontrib>Xie, Haiyang</creatorcontrib><creatorcontrib>Zheng, Shusen</creatorcontrib><creatorcontrib>Wang, Weilin</creatorcontrib><creatorcontrib>Zhou, Dongkai</creatorcontrib><creatorcontrib>Zhang, Linshi</creatorcontrib><creatorcontrib>Yan, Yingcai</creatorcontrib><creatorcontrib>Zhou, Xiaohu</creatorcontrib><creatorcontrib>He, Tianyu</creatorcontrib><creatorcontrib>Zhang, Lufei</creatorcontrib><creatorcontrib>Kong, Yang</creatorcontrib><creatorcontrib>Huang, Yu</creatorcontrib><creatorcontrib>Zhou, Jiarong</creatorcontrib><title>High Expression of ITGA3 Promotes Proliferation and Cell Cycle Progression and Indicates Poor Prognosis in Intrahepatic Cholangiocarcinoma</title><title>BioMed research international</title><addtitle>Biomed Res Int</addtitle><description>Integrin subunit alpha 3 (ITGA3) interacts with a beta 1 subunit to form a member of the integrin family. Integrins are heterodimeric integral membrane proteins that serve as cell surface adhesion proteins. In this research, we investigated the biological function of this protein in human intrahepatic cholangiocarcinoma (ICC) for the first time. Here, using Western blotting and immunohistochemistry assays, we discovered that ITGA3 was overexpressed in ICC cell lines and ICC patients. Moreover, we found ITGA3 expression correlated with several clinicopathological features, including tumor size, lymph node metastasis, and the TNM stage. Patients with high ITGA3 expression underwent a worse prognosis after complete resection compared with patients with low ITGA3 expression in terms of overall survival. Furthermore, we demonstrated that ITGA3 could significantly promote ICC cell proliferation and cell cycle progression in vitro. 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Integrins are heterodimeric integral membrane proteins that serve as cell surface adhesion proteins. In this research, we investigated the biological function of this protein in human intrahepatic cholangiocarcinoma (ICC) for the first time. Here, using Western blotting and immunohistochemistry assays, we discovered that ITGA3 was overexpressed in ICC cell lines and ICC patients. Moreover, we found ITGA3 expression correlated with several clinicopathological features, including tumor size, lymph node metastasis, and the TNM stage. Patients with high ITGA3 expression underwent a worse prognosis after complete resection compared with patients with low ITGA3 expression in terms of overall survival. Furthermore, we demonstrated that ITGA3 could significantly promote ICC cell proliferation and cell cycle progression in vitro. However, as a classical cell surface adhesion molecule, we found ITGA3 correlated negatively with the migration and invasion of ICC cell lines, which differs from other malignant tumors. Generally, these findings suggest that ITGA3 may play a role as a potential oncogene in ICC and suppression of ITGA3 expression may establish a novel target for guiding the therapy of ICC patients.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>29511671</pmid><doi>10.1155/2018/2352139</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0003-1459-8261</orcidid><orcidid>https://orcid.org/0000-0001-9432-2649</orcidid><orcidid>https://orcid.org/0000-0002-6270-0737</orcidid><oa>free_for_read</oa></addata></record>
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subjects Adhesion
Biotechnology
Cancer therapies
Cell culture
Cell cycle
Cell proliferation
Cell surface
Cholangiocarcinoma
Extracellular matrix
Hospitals
Immunohistochemistry
Integral membrane proteins
Integrins
Laboratories
Liver cancer
Lymph nodes
Medical diagnosis
Medical prognosis
Medicine
Melanoma
Membrane proteins
Metastases
Metastasis
MicroRNAs
Patients
Prognosis
Prostate cancer
Proteins
Rodents
Surgery
Surgical outcomes
Tumors
Western blotting
title High Expression of ITGA3 Promotes Proliferation and Cell Cycle Progression and Indicates Poor Prognosis in Intrahepatic Cholangiocarcinoma
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