Antitumor activity of antibody against cytotoxic T lymphocyte epitope peptide of lymphocyte‐specific protein tyrosine kinase

• Published in: Cancer science 2018-03, Vol.109 (3), p.611-617
• Main Authors: Matsueda, Satoko, Itoh, Kyogo, Shichijo, Shigeki
• Format: Article
• Language: English
• Subjects: Animal models; Animals; Antigens; Antineoplastic Agents, Immunological - administration & dosage; Antineoplastic Agents, Immunological - pharmacology; Antitumor activity; anti‐Lck‐486 peptide antibody; Biological activity; Bone marrow; Cancer; cancer vaccine; Cancer vaccines; Cell Line, Tumor; Cloning; Colonic Neoplasms - drug therapy; Colonic Neoplasms - immunology; Cytotoxicity; Dendritic cells; Dendritic Cells - drug effects; Epitopes; Epitopes, T-Lymphocyte - immunology; Experiments; Humans; Immunoglobulins; Immunoregulation; Immunotherapy; Kinases; Lck protein; Lymphocyte Specific Protein Tyrosine Kinase p56(lck) - chemistry; Lymphocyte Specific Protein Tyrosine Kinase p56(lck) - immunology; Lymphocytes; Lymphocytes T; lymphocyte‐specific protein tyrosine kinase; Metastasis; Mice; Monoclonal antibodies; Original; Peptides; Protein-tyrosine kinase; Proteins; T-Lymphocytes, Cytotoxic - drug effects; T-Lymphocytes, Cytotoxic - immunology; T‐cell epitope peptide; Vaccination; Vaccines; Xenograft Model Antitumor Assays
• ISSN: 1347-9032; 1349-7006
• DOI: 10.1111/cas.13522

Although humoral responses against CTL epitope peptides from lymphocyte‐specific protein tyrosine kinase (Lck) antigen have been observed in the majority of healthy donors and cancer patients, the biological activity of the antibody has not been determined. We investigated the biological activity of mAb against CTL epitope peptide of Lck antigen at positions 486‐494 (anti‐Lck‐486 mAb). This mAb induced dendritic cell maturation from murine bone marrow cells by the immune complex form in vitro, and inhibited tumor growth in association with a suppression of tumor‐infiltrating T cells, including T regulatory cells in a murine model using female BALB/cCrlCrlj mice (H‐2Kd). More potent tumor inhibition was observed when this mAb was given prior to peptide vaccination. These results may help to unveil the biological activity of anti‐Lck peptide antibodies against CTL epitope peptides. Anti‐Lck486 monoclonal antibody induced dendritic cell maturation from murine bone marrow cells in vitro, and inhibited tumor growth in association with a suppression of tumor infiltrating T cells in a murine model using the BALB/cCrlCrlj mice. More potent tumor inhibition was observed when this monoclonal antibody was administered prior to the peptide vaccination.