D-alanyl-D-alanine-Modified Gold Nanoparticles Form a Broad-Spectrum Sensor for Bacteria

Rapid and facile detection of pathogenic bacteria is challenging due to the requirement of large-scale instruments and equipment in conventional methods. We utilize D-amino acid as molecules to selectively target bacteria because bacteria can incorporate DADA in its cell wall while mammalian cells o...

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Bibliographic Details
Published in:Theranostics 2018-01, Vol.8 (5), p.1449-1457
Main Authors: Yang, Xinglong, Dang, Yan, Lou, Jinli, Shao, Huawu, Jiang, Xingyu
Format: Article
Language:English
Subjects:
Ascites - diagnosis
Ascites - microbiology
Cell Death
Colorimetry
Dipeptides - chemical synthesis
Dipeptides - chemistry
Gold - chemistry
HeLa Cells
Human Umbilical Vein Endothelial Cells - cytology
Humans
Metal Nanoparticles - chemistry
Metal Nanoparticles - ultrastructure
Research Paper
Staphylococcus aureus - cytology
Staphylococcus aureus - ultrastructure
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Summary:Rapid and facile detection of pathogenic bacteria is challenging due to the requirement of large-scale instruments and equipment in conventional methods. We utilize D-amino acid as molecules to selectively target bacteria because bacteria can incorporate DADA in its cell wall while mammalian cells or fungi cannot. We show a broad-spectrum bacterial detection system based on D-amino acid-capped gold nanoparticles (AuNPs). AuNPs serve as the signal output that we can monitor without relying on any complex instruments. In the presence of bacteria, the AuNPs aggregate and the color of AuNPs changes from red to blue. This convenient color change can distinguish between ( ) and methicillin-resistant (MRSA). This system can be applied for detection of ascites samples from patients. These D-amino acid-modified AuNPs serve as a promising platform for rapid visual identification of pathogens in the clinic.
ISSN:1838-7640
1838-7640
DOI:10.7150/thno.22540