Exposure of kale root to NaCl and Na2SeO3 increases isothiocyanate levels and Nrf2 signalling without reducing plant root growth

A plant factory is a closed cultivation system that provides a consistent and modified environment for plant growth. We speculated that treatment of kale ( Brassica oleracea ) grown in a plant factory with NaCl, Na 2 SeO 3 , or both would increase the bioactive phytochemical levels including glucosi...

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Bibliographic Details
Published in:Scientific reports 2018-03, Vol.8 (1), p.1-11, Article 3999
Main Authors: Kim, Sun Young, Park, Jai-Eok, Kim, Eun Ok, Lim, Sue Ji, Nam, Eui Jeong, Yun, Ji Ho, Yoo, GyHye, Oh, Sang-Rok, Kim, Hyoung Seok, Nho, Chu Won
Format: Article
Language:English
Subjects:
38/1
631/449/2667
631/67/2195
Antioxidants
Biological activity
Brassica oleracea
Cancer
Glucosinolates
Hepatoma
Humanities and Social Sciences
Isothiocyanate
multidisciplinary
Plant cells
Plant extracts
Plant growth
Science
Science (multidisciplinary)
Sinigrin
Sodium chloride
Vegetables
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Summary:A plant factory is a closed cultivation system that provides a consistent and modified environment for plant growth. We speculated that treatment of kale ( Brassica oleracea ) grown in a plant factory with NaCl, Na 2 SeO 3 , or both would increase the bioactive phytochemical levels including glucosinolates (GLSs) and isothiocyanates (ITCs), the key molecules in cancer prevention. The kale was harvested and analysed after treatment with NaCl and Na 2 SeO 3 alone or in combination for 1 or 2 weeks. Exposure to NaCl alone but not Na 2 SeO 3 increased plant root growth. Levels of sinigrin were increased by a 2-week exposure to Na 2 SeO 3 alone or in combination with NaCl, whereas no changes were observed in glucoraphanin and gluconasturtiin gluconasturtiin levels. Importantly, the ITC concentration was affected by 2-week treatment with both compounds. To evaluate the bioactivity of kale, HepG2 human hepatoma cells were treated with plant extract for 6 h. Only the extract of kale roots exposed to a combination NaCl and Na 2 SeO 3 for 2 weeks showed an increased expression of nuclear factor erythroid 2-related factor (Nrf2), which regulates genes encoding antioxidant proteins. These data suggest that co-treatment with NaCl and Na 2 SeO 3 increased the ITC content and chemopreventive effects of kale root.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-018-22411-9