Occurrence of Helicobacter pylori and its major virulence genotypes in dental plaque samples of patients with chronic periodontitis in Iran

• Published in: Gastroenterology and hepatology from bed to bench 2017, Vol.10 (Suppl1), p.S70-S78
• Main Authors: Valadan Tahbaz, Sahel, Yadegar, Abbas, Amirmozafari, Nour, Yaghoobee, Siamak, Ehsani Ardakani, Mohammad Javad, Zojaji, Homayoun
• Format: Article
• Language: English
• Subjects: Original
• ISSN: 2008-2258; 2008-4234
• DOI: 10.22037/ghfbb.v0i0.1267

This study was aimed to investigate the presence of and its virulence genotypes in dental plaques of Iranian patients with chronic periodontitis. is a Gram-negative bacterium that is associated with atrophic gastritis, peptic ulcer, and gastric cancer. Several studies have detected this bacterium in the oral cavity, suggesting it as a potential reservoir. A hundred individuals were divided in 2 groups: 50 patients with chronic periodontitis (case group), and 50 subjects in non-periodontitis (control group). Supragingival and subgingival plaque samples were collected from the individuals using wood wedges and sterile paper points respectively, and prepared for PCR analysis. Totally, DNA was detected in 5 out of 100 (5%) dental plaques. Of 5 dental plaques positive for , gene was detected in 4 specimen, 3 in periodontitis group and one in non-periodontitis group. The s1m1 genotype was predominantly detected in 2/5 samples. The gene was detected in all (5/5) -positive dental plaques. There was no significant correlation between the presence of genotypes from dental plaques and chronic periodontitis ( > 0.05). Our results revealed that the rate of is very low in the dental plaques of Iranian patients with chronic periodontitis. Majority of strains from oral cavity were highly virulent based on the main clinically virulence factors they carried.