Curcumin may serve an anticancer role in human osteosarcoma cell line U-2 OS by targeting ITPR1

The present study aimed to determine the mechanisms of action of curcumin in osteosarcoma. Human osteosarcoma U-2 OS cells was purchased from the Cell Bank of the Chinese Academy of Sciences. RNA sequencing analysis was performed for 2 curcumin-treated samples and 2 control samples using Illumina de...

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Bibliographic Details
Published in:Oncology letters 2018-04, Vol.15 (4), p.5593-5601
Main Authors: Luo, Zhanpeng, Li, Dawei, Luo, Xiaobo, Li, Litao, Gu, Suxi, Yu, Long, Ma, Yuanzheng
Format: Article
Language:English
Subjects:
Apoptosis
Bone cancer
Care and treatment
Curcumin
Development and progression
Genes
Genetic aspects
Health aspects
Hypoxia
Innovations
Kinases
Metabolism
Molecular targeted therapy
Oncology
Ontology
Osteosarcoma
Polypeptides
Proteins
Quality control
Sarcoma
Signal transduction
Software
Transcription factors
Transport proteins
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Summary:The present study aimed to determine the mechanisms of action of curcumin in osteosarcoma. Human osteosarcoma U-2 OS cells was purchased from the Cell Bank of the Chinese Academy of Sciences. RNA sequencing analysis was performed for 2 curcumin-treated samples and 2 control samples using Illumina deep sequencing technology. The differentially expressed genes were identified using Cufflink software. Enrichment and protein-protein interaction network analyses were performed separately using cluster Profiler package and Cytoscape software to identify key genes. Then, the mRNA levels of key genes were detected by quantitative reverse transcription polymerase chain reaction (RT-qPCR) in U-2 OS cells. Finally, cell apoptosis, proliferation, migration and invasion arrays were performed. In total, 201 DEGs were identified in the curcumin-treated group. EEF1A1 (degree=88), ATF7IP, HIF1A, SMAD7, CLTC, MCM10, ITPR1, ADAM15, WWP2 and ATP5C1, which were enriched in 'biological process', exhibited higher degrees than other genes in the PPI network. RT-qPCR demonstrated that treatment with curcumin was able to significantly increase the levels of CLTC and ITPR1 mRNA in curcumin-treated cells compared with control. In addition, targeting ITPR1 with curcumin significantly promoted apoptosis and suppressed proliferation, migration and invasion. Targeting ITPR1 via curcumin may serve an anticancer role by mediating apoptosis, proliferation, migration and invasion in U-2 OS cells.
ISSN:1792-1074
1792-1082
DOI:10.3892/ol.2018.8032