Establishment and Characterization of a High and Stable Porcine CD163-Expressing MARC-145 Cell Line

Isolation and identification of diverse porcine reproductive and respiratory syndrome viruses (PRRSVs) play a fundamental role in PRRSV research and disease management. However, PRRSV has a restricted cell tropism for infection. MARC-145 cells are routinely used for North American genotype PRRSV iso...

Full description

Saved in:
Bibliographic Details
Published in:BioMed research international 2018-01, Vol.2018 (2018), p.1-9
Main Authors: Li, Feng, Sun, Wenbo, Guo, Lihui, Wu, Jiaqiang, Li, Jun, Wang, Jinbao, Zhao, Xiaomin, Du, Yijun, Tian, Fulin, Wang, Guisheng, Yoo, Dongwan, Hu, Yue, Chen, Lei, Cong, Xiaoyan, Qi, Jing, Wu, Xiangju, Chen, Zhi
Format: Article
Language:English
Subjects:
Archives & records
CD163 antigen
Cell lines
Disease control
Disease susceptibility
Genes
Genotype & phenotype
Genotypes
Hogs
Immunofluorescence
Infection
Infections
Medical research
Medicine, Experimental
Mortality
Penicillin
Tropism
Vaccines
Virology
Viruses
Western blotting
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Isolation and identification of diverse porcine reproductive and respiratory syndrome viruses (PRRSVs) play a fundamental role in PRRSV research and disease management. However, PRRSV has a restricted cell tropism for infection. MARC-145 cells are routinely used for North American genotype PRRSV isolation and vaccine production. But MARC-145 cells have some limitations such as low virus yield. CD163 is a cellular receptor that mediates productive infection of PRRSV in various nonpermissive cell lines. In this study, we established a high and stable porcine CD163- (pCD163-) expressing MARC-145 cell line toward increasing its susceptibility to PRRSV infection. Indirect immunofluorescence assay (IFA) and Western blotting assays showed that pCD163 was expressed higher in pCD163-MARC cell line than MARC-145 cells. Furthermore, the ability of pCD163-MARC cell line to propagate PRRSV was significantly increased as compared with MARC-145 cells. Finally, we found that pCD163-MARC cell line had a higher isolation rate of clinical PRRSV samples and propagated live attenuated PRRS vaccine strains more efficiently than MARC-145 cells. This pCD163-MARC cell line will be a valuable tool for propagation and research of PRRSV.
ISSN:2314-6133
2314-6141
DOI:10.1155/2018/4315861