Effect of antibiotics and NSAIDs on cyclooxygenase-2 in the enamel mineralization

The objective of this study was to determine whether the use of the most commonly prescribed antibiotics and non-steroidal anti-inflammatory drugs in childhood could disturb enamel mineralization. Forty-two Swiss mice were divided into seven groups: controls; amoxicillin; amoxicillin/clavulanate; er...

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Bibliographic Details
Published in:Scientific reports 2018-03, Vol.8 (1), p.4132-7, Article 4132
Main Authors: Serna Muñoz, Clara, Pérez Silva, Amparo, Solano, Francisco, Castells, María Teresa, Vicente, Ascensión, Ortiz Ruiz, Antonio José
Format: Article
Language:English
Subjects:
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Acetaminophen
Aluminum
Amoxicillin
Analgesics
Anti-inflammatory agents
Antibiotics
Calcium
Celecoxib
Chemical elements
Children
Chlorine
Cyclooxygenase-2
Dental enamel
Enamel
Erythromycin
Humanities and Social Sciences
Ibuprofen
Image processing
Incisors
Inflammation
Magnesium
Mineralization
multidisciplinary
Nonsteroidal anti-inflammatory drugs
Phosphorus
Science
Science (multidisciplinary)
Sodium
Teeth
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Summary:The objective of this study was to determine whether the use of the most commonly prescribed antibiotics and non-steroidal anti-inflammatory drugs in childhood could disturb enamel mineralization. Forty-two Swiss mice were divided into seven groups: controls; amoxicillin; amoxicillin/clavulanate; erythromycin; acetaminophen; ibuprofen and celecoxib, to inhibit cyclooxygenase 2 (COX2). SEM-EDX analysis was conducted on all cusps of the third molars. Calcium (Ca), phosphorus (P), aluminum, potassium, sodium, magnesium and chlorine were quantified. The stoichiometric Ca/P molar ratios were calculated. Immunohistochemical quantification of COX2 in incisors was carried out by image analysis using COX2-specific immunostaining. Groups treated with antibiotics showed no significant differences in the content of the chemical elements. Only acetaminophen and celecoxib showed a significant decrease in Ca and P compared with the control samples. Ca/P ratios showed no difference. Groups treated with amoxicillin, amoxicillin/clavulanate, erythromycin and acetaminophen showed significantly lower amounts of immunoreactive COX2 at the enamel organ maturation stage of the mouse incisors. Our results suggest that COX2 is involved in the maturation stage of the enamel organ and that its inhibition would appear to alter amelogenesis, producing hypomineralization.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-018-22607-z