2-aminopurine suppresses the TGF-β1-induced epithelial–mesenchymal transition and attenuates bleomycin-induced pulmonary fibrosis

• Published in: Cell death discovery 2018-12, Vol.4 (1), p.17-10, Article 17
• Main Authors: Weng, Dong, Chen, Jian-xia, Li, Hao-hao, Liu, Feng, Zhou, Li-dan, Liu, Hai-peng, Zheng, Rui-juan, Jiang, Yan, Liu, Zhong-hua, Ge, Baoxue
• Format: Article
• Language: English
• Subjects: 2-Aminopurine; 631/154/1435/2163; 692/308/575; Adenosine; Apoptosis; Biochemistry; Biomedical and Life Sciences; Bleomycin; Cell Biology; Cell Cycle Analysis; Collagen; E-cadherin; Epithelial cells; Fibroblasts; Fibronectin; Fibrosis; Guanosine; Life Sciences; Lung cancer; Lung diseases; Mesenchyme; Metastases; Metastasis; Pulmonary fibrosis; Stem Cells; Transforming growth factor-b1; Vimentin
• ISSN: 2058-7716; 2058-7716
• DOI: 10.1038/s41420-017-0016-3

The epithelial–mesenchymal transition (EMT) is a multifunctional cell process involved in the pathogenesis of numerous conditions, including fibrosis and cancer. Idiopathic pulmonary fibrosis (IPF) is a progressive and fatal disease characterized by fibroblast accumulation and collagen deposition in the lungs. The fibroblasts involved in this process partially originate from lung epithelial cells via the EMT. Evidence suggests that the EMT contributes to progression, invasion, and metastasis of various types of cancer. We screened a series of 80 compounds for the ability to interfere with the EMT and potentially be applied as a therapeutic for IPF and/or lung cancer. We identified 2-aminopurine (2-AP), a fluorescent analog of guanosine and adenosine, as a candidate in this screen. Herein, we demonstrate that 2-AP can restore E-cadherin expression and inhibit fibronectin and vimentin expression in TGF-β1–treated A549 lung cancer cells. Moreover, 2-AP can inhibit TGF-β1-induced metastasis of A549 cells. This compound significantly attenuated bleomycin (BLM)-induced pulmonary inflammation, the EMT, and fibrosis. In addition, 2-AP treatment significantly decreased mortality in a mouse model of pulmonary fibrosis. Collectively, we determined that 2-AP could inhibit metastasis in vitro by suppressing the TGF-β1-induced EMT and could attenuate BLM-induced pulmonary fibrosis in vivo. Results of this study suggest that 2-AP may have utility as a treatment for lung cancer and pulmonary fibrosis.