Optimization of Ex Vivo Murine Bone Marrow Derived Immature Dendritic Cells: A Comparative Analysis of Flask Culture Method and Mouse CD11c Positive Selection Kit Method

12–14 days of culturing of bone marrow (BM) cells containing various growth factors is widely used method for generating dendritic cells (DCs) from suspended cell population. Here we compared flask culture method and commercially available CD11c Positive Selection kit method. Immature BMDCs’ purity...

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Bibliographic Details
Published in:Bone marrow research 2018-01, Vol.2018, p.3495086-9
Main Authors: Gosavi, Rahul Ashok, Salwe, Sukeshani, Mukherjee, Sandeepan, Dahake, Ritwik, Kothari, Sweta, Patel, Vainav, Chowdhary, Abhay, Deshmukh, Ranjana A.
Format: Article
Language:English
Subjects:
Analysis
Antigens
Bone marrow
Bone marrow cells
Cell culture
Cloning
Cytokines
Dendritic cells
Genetic aspects
Granulocyte-macrophage colony stimulating factor
Granulocytes
Growth factors
Immunology
Lymphocytes
Methods
Physiological aspects
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Summary:12–14 days of culturing of bone marrow (BM) cells containing various growth factors is widely used method for generating dendritic cells (DCs) from suspended cell population. Here we compared flask culture method and commercially available CD11c Positive Selection kit method. Immature BMDCs’ purity of adherent as well as suspended cell population was generated in the decreasing concentration of recombinant-murine granulocyte-macrophage colony-stimulating factor (rmGM-CSF) in nontreated tissue culture flasks. The expression of CD11c, MHCII, CD40, and CD86 was measured by flow cytometry. We found significant difference (P
ISSN:2090-2999
2090-3006
DOI:10.1155/2018/3495086