miR-205 mediates the inhibition of cervical cancer cell proliferation using olmesartan

Objective: The renin-angiotensin-aldosterone system has become known as a prerequisite for tumor angiogenesis that is now recognized as a crucial step in the development of tumors, including cervical cancer. The Ang II-AT1R pathway is known to play an important role in tumor angiogenesis. MicroRNAs...

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Published in:Journal of the renin-angiotensin-aldosterone system 2016-07, Vol.17 (3), p.1470320316663327-1470320316663327
Main Authors: Yue, Zhang, Yun-shan, Zhang, Feng-xia, Xue
Format: Article
Language:English
Subjects:
Angiotensin II
Base Sequence
Cell Line, Tumor
Cell Proliferation - drug effects
Drug Resistance, Neoplasm - drug effects
Drug Resistance, Neoplasm - genetics
Female
Gene Expression Regulation, Neoplastic - drug effects
Humans
Imidazoles - pharmacology
MicroRNAs - genetics
MicroRNAs - metabolism
Neoplasm Invasiveness
Oligonucleotides - metabolism
Original
Tetrazoles - pharmacology
Tumor Stem Cell Assay
Uterine Cervical Neoplasms - genetics
Uterine Cervical Neoplasms - pathology
Vascular Endothelial Growth Factor A - genetics
Vascular Endothelial Growth Factor A - metabolism
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Summary:Objective: The renin-angiotensin-aldosterone system has become known as a prerequisite for tumor angiogenesis that is now recognized as a crucial step in the development of tumors, including cervical cancer. The Ang II-AT1R pathway is known to play an important role in tumor angiogenesis. MicroRNAs (miRNAs) are a class of small, regulating RNAs that participate in tumor genesis, differentiation and proliferation. The current study focused on the anti-tumor mechanism of olmesartan, a novel angiotensin II antagonist, on cervical cancer cells. Materials and methods: qRT-PCR and Western blot were used to demonstrate the effect of olmesartan on miR-205 and VEGF-A expression. miR-205 mimics and VEGF-A shRNA plasmid were separately transfected into HeLa and Siha cells to further validate the function of miR-205 and VEGF-A in cervical cancer cell proliferation. Results: It was found that olmesartan could upregulate miR-205 and inhibit VEGF-A expression in HeLa and Siha cells. In addition, VEGF-A was proven to be a target gene of miR-205. Conclusion: This result provides a new idea on the anti-tumor mechanism of olmesartan, which may be used as a novel therapeutic target of cervical cancer.
ISSN:1470-3203
1752-8976
DOI:10.1177/1470320316663327