Follicle dynamics: visualization and analysis of follicle growth and maturation using murine ovarian tissue culture

Purpose To visualize and analyze follicle development in ovarian tissue culture using physiological concentrations of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) in order to establish an ovarian tissue culture system that enables efficient in vitro growth of follicles. Methods Ov...

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Bibliographic Details
Published in:Journal of assisted reproduction and genetics 2018-02, Vol.35 (2), p.339-343
Main Authors: Murase, Tomohiko, Iwase, Akira, Komatsu, Kouji, Bayasula, Nakamura, Tomoko, Osuka, Satoko, Takikawa, Sachiko, Goto, Maki, Kotani, Tomomi, Kikkawa, Fumitaka
Format: Article
Language:English
Subjects:
Animals
Female
Follicle-stimulating hormone
Follicles
Granulosa Cells
Gynecology
Human Genetics
Luteinizing hormone
Luteinizing Hormone - metabolism
Maturation
Medicine
Medicine & Public Health
Meiosis
Mice, Inbred ICR
Oocytes
Oocytes - physiology
Ovarian Follicle - cytology
Ovarian Follicle - growth & development
Ovarian Follicle - physiology
Ovary - cytology
Reproductive Medicine
Technological Innovations
Time-Lapse Imaging
Tissue culture
Tissue Culture Techniques - methods
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Summary:Purpose To visualize and analyze follicle development in ovarian tissue culture using physiological concentrations of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) in order to establish an ovarian tissue culture system that enables efficient in vitro growth of follicles. Methods Ovarian tissues from 4-week-old female ICR mice were sliced and cultured. Images of ovarian tissues in culture were obtained at 24-h or 30-min intervals by using a microscope. The area of each follicle observed in the ovarian tissue slices was tracked and analyzed in association with oocyte maturation. Results We were able to track the development of each follicle using this culture system. Follicle growth was associated with oocyte maturation. Meiotically matured oocytes (MII) were obtained from 33% of all follicles investigated. Approximately, a quarter of follicles (24%) did not grow and resulted in atresia. Conclusion Follicle dynamics were successfully visualized and analyzed in murine ovarian tissue culture. We were able to obtain mature oocytes from the fully grown follicles in vitro. This culture system would be helpful for efficient in vitro culturing of ovarian tissues.
ISSN:1058-0468
1573-7330
DOI:10.1007/s10815-017-1073-5