The Role of miRNA-132 against Apoptosis and Oxidative Stress in Heart Failure

• Published in: BioMed research international 2018-01, Vol.2018 (2018), p.1-8
• Main Authors: Jin, Hongxu, Hu, Xiaofang, Chen, Keyan, Tong, Zhou, Liu, Xuelei, Hou, Mingxiao
• Format: Article
• Language: English
• Subjects: Alcohol; Animal models; Animals; Antioxidants; Antioxidants - metabolism; Apoptosis; Apoptosis - genetics; Bcl-2 protein; bcl-2-Associated X Protein - genetics; Cardiomyocytes; Cell growth; Disease; Doppler effect; Dyspnea; Edema; Enzyme-linked immunosorbent assay; Female; Fibrosis; Gene expression; Heart; Heart diseases; Heart failure; Heart Failure - genetics; Heart Failure - metabolism; Humans; Hydrogen peroxide; Hypertrophy; Male; Males; Mice; Mice, Inbred C57BL; MicroRNA; MicroRNAs; MicroRNAs - genetics; Middle Aged; miRNA; Myocardium; Myocardium - metabolism; Myocytes, Cardiac - metabolism; Oxidative stress; Oxidative Stress - genetics; Pancreatic cancer; Patients; Peripheral blood; Physiological aspects; Proto-Oncogene Proteins c-bcl-2 - genetics; Ribonucleic acid; RNA; Signal Transduction - genetics; Smad3 protein; Smad3 Protein - genetics; Staining; Transfection; Transforming Growth Factor beta1 - genetics; Transforming growth factor-b1; Transforming growth factors; Ultrasound
• ISSN: 2314-6133; 2314-6141
• DOI: 10.1155/2018/3452748

Objective. To explore the effect of microRNA-132 of heart failure and provide theoretical guidance for clinical treatment of heart failure (HF). Methods. Peripheral blood was collected from HF patients. RT-qPCR was used to determine microRNA-132 expression. Mouse models of heart failure were established. Color Doppler ultrasound was utilized to measure the changes of cardiac function. HE and Masson staining were applied to observe pathological changes of the myocardium. After H9C2 cells were transfected with microRNA-132, MTT assay was employed to detect the stability of H9C2 cells. ELISA was used to measure the levels of oxidative stress factors. Western blot assay and RT-qPCR were utilized to determine the expression of Bax, Bcl-2, TGF-β1, and smad3. Results. MicroRNA-132 expression was downregulated in HF patients’ blood. After establishing mouse models of HF, cardiac function obviously decreased. HE staining revealed the obvious edema and hypertrophy of cardiomyocytes. Masson staining demonstrated that cardiomyocytes were markedly fibrotic. After microRNA-132 transfection and H9C2 cell apoptosis induced by H2O2, antioxidant stress and antiapoptotic ability of the H9C2 cells obviously increased. TGF-β1 and smad3 expression remarkably diminished. Conclusion. Overexpression of microRNA-132 dramatically increased the antioxidant stress and antiapoptotic ability of H9C2 cells and decreased the expression of TGF-β1 and smad3.