Longitudinal Monitoring of Antibody Responses against Tumor Cells Using Magneto-nanosensors with a Nanoliter of Blood

Each immunoglobulin isotype has unique immune effector functions. The contribution of these functions in the elimination of pathogens and tumors can be determined by monitoring quantitative temporal changes in isotype levels. Here, we developed a novel technique using magneto-nanosensors based on th...

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Bibliographic Details
Published in:Nano letters 2017-11, Vol.17 (11), p.6644-6652
Main Authors: Lee, Jung-Rok, Chan, Carmel T, Ruderman, Daniel, Chuang, Hui-Yen, Gaster, Richard S, Atallah, Michelle, Mallick, Parag, Lowe, Scott W, Gambhir, Sanjiv S, Wang, Shan X
Format: Article
Language:English
Subjects:
Animals
Antibody Formation
Biosensing Techniques - instrumentation
Burkitt Lymphoma - blood
Burkitt Lymphoma - diagnostic imaging
Burkitt Lymphoma - immunology
Equipment Design
Female
Immunoglobulin G - analysis
Immunoglobulin G - blood
Immunoglobulin G - immunology
Luminescent Measurements - methods
Magnetics - instrumentation
Mice
Mice, Inbred C57BL
Optical Imaging - instrumentation
Sample Size
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Summary:Each immunoglobulin isotype has unique immune effector functions. The contribution of these functions in the elimination of pathogens and tumors can be determined by monitoring quantitative temporal changes in isotype levels. Here, we developed a novel technique using magneto-nanosensors based on the effect of giant magnetoresistance (GMR) for longitudinal monitoring of total and antigen-specific isotype levels with high precision, using as little as 1 nL of serum. Combining in vitro serologic measurements with in vivo imaging techniques, we investigated the role of the antibody response in the regression of firefly luciferase (FL)-labeled lymphoma cells in spleen, kidney, and lymph nodes in a syngeneic Burkitt’s lymphoma mouse model. Regression status was determined by whole body bioluminescent imaging (BLI). The magneto-nanosensors revealed that anti-FL IgG2a and total IgG2a were elevated and sustained in regression mice compared to non-regression mice (p < 0.05). This platform shows promise for monitoring immunotherapy, vaccination, and autoimmunity.
ISSN:1530-6984
1530-6992
DOI:10.1021/acs.nanolett.7b02591