The Spore Coat Protein CotE Facilitates Host Colonization by Clostridium difficile

Clostridium difficile infection (CDI) is an important hospital-acquired infection resulting from the germination of spores in the intestine as a consequence of antibiotic-mediated dysbiosis of the gut microbiota. Key to this is CotE, a protein displayed on the spore surface and carrying 2 functional...

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Bibliographic Details
Published in:The Journal of infectious diseases 2017-12, Vol.216 (11), p.1452-1459
Main Authors: Hong, Huynh A., Ferreira, William T., Hosseini, Siamand, Anwar, Saba, Hitri, Krisztina, Wilkinson, Anthony J., Vahjen, Wilfried, Zentek, Jürgen, Soloviev, Mikhail, Cutting, Simon M.
Format: Article
Language:English
Subjects:
Adhesins, Bacterial - physiology
Animals
BACTERIA
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Cell Wall - metabolism
Chitinases - metabolism
Clostridioides difficile - genetics
Clostridioides difficile - growth & development
Clostridioides difficile - metabolism
Clostridioides difficile - pathogenicity
Clostridium Infections - microbiology
Colony Count, Microbial
Cricetinae
Disease Models, Animal
Female
Host-Parasite Interactions - physiology
Intestinal Mucosa - microbiology
Intestinal Mucosa - pathology
Major and Brief Reports
Mesocricetus
Mice
Mucins - metabolism
Mutation
Peroxiredoxins - metabolism
Spores, Bacterial - chemistry
Spores, Bacterial - genetics
Spores, Bacterial - growth & development
Spores, Bacterial - pathogenicity
Virulence
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Summary:Clostridium difficile infection (CDI) is an important hospital-acquired infection resulting from the germination of spores in the intestine as a consequence of antibiotic-mediated dysbiosis of the gut microbiota. Key to this is CotE, a protein displayed on the spore surface and carrying 2 functional elements, an N-terminal peroxiredoxin and a C-terminal chitinase domain. Using isogenic mutants, we show in vitro and ex vivo that CotE enables binding of spores to mucus by direct interaction with mucin and contributes to its degradation. In animal models of CDI, we show that when CotE is absent, both colonization and virulence were markedly reduced. We demonstrate here that the attachment of spores to the intestine is essential in the development of CDI. Spores are usually regarded as biochemically dormant, but our findings demonstrate that rather than being simply agents of transmission and dissemination, spores directly contribute to the establishment and promotion of disease.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/jix488