Metabolic profile of danshen in rats by HPLC-LTQ-Orbitrap mass spectrometry

Danshen, the dried root of Salvia miltiorrhiza Bunge (Lamiaceae), is one of the traditional Chinese medicines (TCMs) most commonly used for the treatment of cardiovascular and cerebrovascular diseases. However, little is known about the chemical and metabolic profiles of danshen in vitro or in vivo....

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Bibliographic Details
Published in:Journal of Zhejiang University. B. Science 2018-03, Vol.19 (3), p.227-244
Main Authors: Pang, Huan-huan, Jiang, Mei-fang, Wang, Qin-hui, Wang, Xiao-ye, Gao, Wei, Tian, Zhi-hao, Huang, Jian-mei
Format: Article
Language:English
Subjects:
Bile
Biomedical and Life Sciences
Biomedicine
Cerebrovascular diseases
Ethanol
Formulations
Herbal medicine
High performance liquid chromatography
In vivo methods and tests
Ions
Liquid chromatography
Mass spectrometry
Mass spectroscopy
Medical treatment
Metabolism
Rats
Salvia miltiorrhiza
Tablets
Traditional Chinese medicine
Urine
Vascular diseases
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Summary:Danshen, the dried root of Salvia miltiorrhiza Bunge (Lamiaceae), is one of the traditional Chinese medicines (TCMs) most commonly used for the treatment of cardiovascular and cerebrovascular diseases. However, little is known about the chemical and metabolic profiles of danshen in vitro or in vivo. In particular, more information is needed in relation to the 50% ethanol extracts usually used in danshen formulations such as Fufang Xueshuantong Capsules and Fufang Danshen tablets. High-performance liquid chromatography coupled with a linear ion trap- Orbitrap mass spectrometer (HPLC-LTQ-Orbitrap) provides a sensitive and accurate method for analyzing the composition of samples. This method was used to determine the in vitro and in vivo chemical and metabolic profiles of danshen. Sixty-nine components of danshen extract and 118 components of danshen in rat plasma, urine, feces, and bile were unambiguously or tentatively identified. These results not only revealed the material composition of danshen, but also provided a comprehensive research approach for the identification of multi-constituents in TCMs.
ISSN:1673-1581
1862-1783
DOI:10.1631/jzus.B1700105