Analysis of hedgehog signaling in periocular sebaceous carcinoma

Purpose Sebaceous carcinoma (SC) is a clinical masquerader of benign conditions resulting in significant eye morbidity, sometimes leading to extensive surgical treatment including exenteration, and even mortality. Little is known about the genetic or molecular basis of SC. This study identifies the...

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Bibliographic Details
Published in:Graefe's archive for clinical and experimental ophthalmology 2018-04, Vol.256 (4), p.853-860
Main Authors: Bladen, John C., Moosajee, Mariya, Tracey-White, Dhani, Beaconsfield, Michèle, O’Toole, Edel A., Philpott, Michael P.
Format: Article
Language:English
Subjects:
Basal cell carcinoma
Brain tumors
Eyelid
Glioma
Hedgehog protein
Immunofluorescence
Immunohistochemistry
Kinases
Medicine
Medicine & Public Health
Morbidity
Oncology
Ophthalmology
Patched protein
Physiology
Signal transduction
Stroma
Therapeutic applications
Transcription factors
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Summary:Purpose Sebaceous carcinoma (SC) is a clinical masquerader of benign conditions resulting in significant eye morbidity, sometimes leading to extensive surgical treatment including exenteration, and even mortality. Little is known about the genetic or molecular basis of SC. This study identifies the involvement of Hedgehog (Hh) signaling in periocular SC. Methods Fifteen patients with periocular SC patients were compared to 15 patients with eyelid nodular basal cell carcinoma (nBCC; a known Hh tumor), alongside four normal individuals as a control for physiological Hh expression. Expression of Patched 1 (PTCH1), Smoothened (SMO), and glioma-associated zinc transcription factors (Gli1 and Gli2) were assessed in histological sections using immunohistochemistry and immunofluorescence (IF) techniques. Antibody specificity was verified using Western-blot analysis of a Gli1 over-expressed cancer cell line, LNCaP-Gli1. Semi-quantification compared tumors and control tissue using IF analysis by ImageJ software. Results Expression of the Hh pathway was observed in SC for all four major components of the pathway. PTCH1, SMO, and Gli2 were more significantly upregulated in SC ( P  
ISSN:0721-832X
1435-702X
DOI:10.1007/s00417-018-3900-5