Nucleotide-mediated SPDEF modulates TFF3-mediated wound healing and intestinal barrier function during the weaning process

Most alterations during weaning involve physiological changes in intestinal structure and function. Here, we evaluated the molecular mechanisms regulating the effects of nucleotides on weaning. Nucleotide treatment induced Trefoil factor 3 (TFF3) expression and IPEC-J2 cell growth and reduced wound...

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Bibliographic Details
Published in:Scientific reports 2018-03, Vol.8 (1), p.4827-13, Article 4827
Main Authors: Lee, Sang In, Kim, In Ho
Format: Article
Language:English
Subjects:
1-Phosphatidylinositol 3-kinase
631/337/2019
631/601/1737
AKT protein
ETS protein
Extracellular signal-regulated kinase
Humanities and Social Sciences
Intestine
Janus kinase
Lipopolysaccharides
Molecular modelling
multidisciplinary
Nucleosides
Nucleotides
Permeability
Science
Science (multidisciplinary)
Small intestine
Structure-function relationships
Supplements
Trefoil factor
Weaning
Wound healing
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Summary:Most alterations during weaning involve physiological changes in intestinal structure and function. Here, we evaluated the molecular mechanisms regulating the effects of nucleotides on weaning. Nucleotide treatment induced Trefoil factor 3 (TFF3) expression and IPEC-J2 cell growth and reduced wound width. Treatment with nucleosides and TFF3 in lipopolysaccharide-challenged IPEC-J2 cells increased intestinal transepithelial electrical resistance and decreased intestinal permeability. Additionally, nucleosides improved intestinal barrier function through induction of TFF3-mediated phosphatidylinositol 3-kinase/Akt, extracellular signal-regulated kinase 1/2, p38, and Janus kinase/signal transducer and activator of transcription signaling pathways. Among selected differentially expressed genes, SAM pointed domain containing ETS transcription factor (SPDEF) expression was elevated by nucleotides in a concentration-dependent manner. Moreover, SPDEF directly regulated TFF3 expression via binding to the promoter. In vivo , nucleotide supplementation improved growth performance, serum stress levels, and intestinal morphology. Our findings provide insights into the molecular mechanisms of intestinal development during weaning in pigs.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-018-23218-4