Gender differences in the regulation of MLC20 phosphorylation and smooth muscle contraction in rat stomach

Evidence of sex-related differences in gastrointestinal (GI) functions has been reported in the literature. In addition, various GI disorders have disproportionate prevalence between the sexes. An essential step in the initiation of smooth muscle contraction is the phosphorylation of the 20-kDa regu...

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Published in:Biomedical reports 2018-03, Vol.8 (3), p.283-288
Main Authors: Al-Shboul, Othman A, Al-Dwairi, Ahmed N, Alqudah, Mohammad A, Mustafa, Ayman G
Format: Article
Language:English
Subjects:
Acetylcholine receptors (muscarinic)
Adenosine
Calcium ions
Calcium-binding protein
Calmodulin
Chains
Enzyme-linked immunosorbent assay
Enzymes
Esophagus
Experiments
Fecal incontinence
Females
Gender aspects
Gender differences
Kinases
Laboratories
Males
Motility
Muscle contraction
Muscles
Myosin
Myosin-light-chain kinase
Phosphatase
Phosphorylation
Proteins
Rats
Rodents
Sex
Sex differences
Signaling
Smooth muscle
Stomach
Studies
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cited_by cdi_FETCH-LOGICAL-c389t-4669920eebe8c8878628c5478d7773dc954bd984a441f0d13ab2515a28417dea3
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creator Al-Shboul, Othman A
Al-Dwairi, Ahmed N
Alqudah, Mohammad A
Mustafa, Ayman G
description Evidence of sex-related differences in gastrointestinal (GI) functions has been reported in the literature. In addition, various GI disorders have disproportionate prevalence between the sexes. An essential step in the initiation of smooth muscle contraction is the phosphorylation of the 20-kDa regulatory myosin light chain (MLC20) by the Ca2+/calmodulin-dependent myosin light chain kinase (MLCK). However, whether male stomach smooth muscle inherits different contractile signaling mechanisms for the regulation of MLC20 phosphorylation from that in females has not been established. The present study was designed to investigate sex-associated differences in the regulation of MLC20 phosphorylation and thus muscle contraction in gastric smooth muscle cells (GSMCs). Experiments were performed on GSMCs freshly isolated from male and female rats. Contraction of the GSMCs in response to acetylcholine (ACh), a muscarinic agonist, was measured via scanning micrometry in the presence or absence of the MLCK inhibitor, ML-7. Additionally, the protein levels of MLC20, MLCK and phosphorylated MLC20 were measured by ELISA. The protein levels of MLC20 and MLCK were indifferent between the sexes. ACh induced greater contraction (P
doi_str_mv 10.3892/br.2018.1053
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In addition, various GI disorders have disproportionate prevalence between the sexes. An essential step in the initiation of smooth muscle contraction is the phosphorylation of the 20-kDa regulatory myosin light chain (MLC20) by the Ca2+/calmodulin-dependent myosin light chain kinase (MLCK). However, whether male stomach smooth muscle inherits different contractile signaling mechanisms for the regulation of MLC20 phosphorylation from that in females has not been established. The present study was designed to investigate sex-associated differences in the regulation of MLC20 phosphorylation and thus muscle contraction in gastric smooth muscle cells (GSMCs). Experiments were performed on GSMCs freshly isolated from male and female rats. Contraction of the GSMCs in response to acetylcholine (ACh), a muscarinic agonist, was measured via scanning micrometry in the presence or absence of the MLCK inhibitor, ML-7. Additionally, the protein levels of MLC20, MLCK and phosphorylated MLC20 were measured by ELISA. The protein levels of MLC20 and MLCK were indifferent between the sexes. ACh induced greater contraction (P&lt;0.05) as well as greater MLC20 phosphorylation (P&lt;0.05) in male GSMCs compared with female. Pretreatment of GSMCs with ML-7 significantly reduced the ACh-induced contraction (P&lt;0.05) and MLC20 phosphorylation (P&lt;0.05) in the male and female cells, and notably, abolished the contractile differences between the sexes. In conclusion, MLC20 phosphorylation and thus muscle contraction may be activated to a greater extent in male rat stomach compared with that in females.</description><identifier>ISSN: 2049-9434</identifier><identifier>EISSN: 2049-9442</identifier><identifier>DOI: 10.3892/br.2018.1053</identifier><identifier>PMID: 29599980</identifier><language>eng</language><publisher>Athens: Spandidos Publications UK Ltd</publisher><subject>Acetylcholine receptors (muscarinic) ; Adenosine ; Calcium ions ; Calcium-binding protein ; Calmodulin ; Chains ; Enzyme-linked immunosorbent assay ; Enzymes ; Esophagus ; Experiments ; Fecal incontinence ; Females ; Gender aspects ; Gender differences ; Kinases ; Laboratories ; Males ; Motility ; Muscle contraction ; Muscles ; Myosin ; Myosin-light-chain kinase ; Phosphatase ; Phosphorylation ; Proteins ; Rats ; Rodents ; Sex ; Sex differences ; Signaling ; Smooth muscle ; Stomach ; Studies</subject><ispartof>Biomedical reports, 2018-03, Vol.8 (3), p.283-288</ispartof><rights>Copyright Spandidos Publications UK Ltd. 2018</rights><rights>Copyright © 2019, Spandidos Publications 2019</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c389t-4669920eebe8c8878628c5478d7773dc954bd984a441f0d13ab2515a28417dea3</citedby><cites>FETCH-LOGICAL-c389t-4669920eebe8c8878628c5478d7773dc954bd984a441f0d13ab2515a28417dea3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5867467/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5867467/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids></links><search><creatorcontrib>Al-Shboul, Othman A</creatorcontrib><creatorcontrib>Al-Dwairi, Ahmed N</creatorcontrib><creatorcontrib>Alqudah, Mohammad A</creatorcontrib><creatorcontrib>Mustafa, Ayman G</creatorcontrib><title>Gender differences in the regulation of MLC20 phosphorylation and smooth muscle contraction in rat stomach</title><title>Biomedical reports</title><description>Evidence of sex-related differences in gastrointestinal (GI) functions has been reported in the literature. In addition, various GI disorders have disproportionate prevalence between the sexes. An essential step in the initiation of smooth muscle contraction is the phosphorylation of the 20-kDa regulatory myosin light chain (MLC20) by the Ca2+/calmodulin-dependent myosin light chain kinase (MLCK). However, whether male stomach smooth muscle inherits different contractile signaling mechanisms for the regulation of MLC20 phosphorylation from that in females has not been established. The present study was designed to investigate sex-associated differences in the regulation of MLC20 phosphorylation and thus muscle contraction in gastric smooth muscle cells (GSMCs). Experiments were performed on GSMCs freshly isolated from male and female rats. Contraction of the GSMCs in response to acetylcholine (ACh), a muscarinic agonist, was measured via scanning micrometry in the presence or absence of the MLCK inhibitor, ML-7. 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ispartof Biomedical reports, 2018-03, Vol.8 (3), p.283-288
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2049-9442
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recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5867467
source PubMed (Medline)
subjects Acetylcholine receptors (muscarinic)
Adenosine
Calcium ions
Calcium-binding protein
Calmodulin
Chains
Enzyme-linked immunosorbent assay
Enzymes
Esophagus
Experiments
Fecal incontinence
Females
Gender aspects
Gender differences
Kinases
Laboratories
Males
Motility
Muscle contraction
Muscles
Myosin
Myosin-light-chain kinase
Phosphatase
Phosphorylation
Proteins
Rats
Rodents
Sex
Sex differences
Signaling
Smooth muscle
Stomach
Studies
title Gender differences in the regulation of MLC20 phosphorylation and smooth muscle contraction in rat stomach
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