Role of offending out-door aero-allergen and CD14 C(-159)T polymorphism in development and severity of asthma in a Kolkata patient population

• Published in: African health sciences 2017-12, Vol.17 (4), p.1101-1109
• Main Authors: Dutta, Shampa, Mondal, Priti, Saha, Nimai Chandra, Moitra, Saibal, Podder, Sanjoy, Ghosh, Amlan, Saha, Goutam Kumar
• Format: Article
• Language: English
• Subjects: Adolescent; Allergens - immunology; Asthma - blood; Asthma - genetics; Asthma - immunology; Biomarkers - blood; Case-Control Studies; Child; Female; Genetic Predisposition to Disease; Humans; Hypersensitivity, Immediate; Immunoglobulin E - blood; Immunoglobulin E - immunology; Lipopolysaccharide Receptors - genetics; Male; Polymorphism, Genetic; Polymorphism, Single Nucleotide; Promoter Regions, Genetic - genetics; Promoter Regions, Genetic - immunology; Skin Tests; Young Adult
• ISSN: 1680-6905; 1729-0503; 1680-6905
• DOI: 10.4314/ahs.v17i4.18

Present study involved identification of offending out-door aero-allergens and associated genetic pathway in nasso-bronchial asthma among Kolkata population. Skin-prick test was done among 950 asthmatic patients against 11 common aero-allergens and total serum IgE concentration was measured. PCR-RFLP was done in patients and non-asthmatic control (n=220 in each) to characterize functional polymorphism, C(-159)T, of CD14, a positional candidate gene for allergy. Association of genetic polymorphisms was made with clinico-pathological conditions. We identified as the most common aero-allergen sensitizer among atopic patients in Kolkata. Patients with childhood-onset asthma were significantly more sensitive towards aero-allergens and had significantly higher serum IgE level than those of adult-onset (p< 0.0001). No significant difference was found in distribution of SNP genotypes of CD14 among case and control (p=0.178). However among patients, frequency of C allele is significantly higher in childhood-onset group than that of adult-onset and concordantly in former CC genotype was associated with significant higher level of serum IgE than CT and TT. In Kolkata, pollen is a common out-door aero-allergen and is predominant among pollens. Childhood-onset and adult-onset of asthma showed significant difference in allergen sensitivity as well as genetic background with respect to CD14 polymorphism.